Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum

Yuriko Osakabe; Keishi Osakabe; Vincent L. Chiang

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Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum

机译：烟草中烟叶松（Pinus taeda）苯丙氨酸解氨酶基因启动子的组织特异性表达的表征

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We isolated the 5′ flanking region of a gene for phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) from Pinus taeda, PtaPAL. To investigate the tissue-specific expression of the PtaPAL promoter, histochemical assay of GUS activity was performed using the transgenic tobacco expressing the PtaPAL promoter-GUS. The region of −897 to −420 in PtaPAL promoter showed high activities in the secondary xylem and response to bending stress. To characterize the cis-regulatory functions of the promoters for enzymes in phenylpropanoid biosynthesis, we examined the activity of chimeric promoters of PtaPAL and a 4-coumarate CoA ligase, Pta4CLα. The chimeric promoter showed similar activity as the Pta4CLα promoter. Electrophoretic mobility shift assays implicated −897 to –674 of PtaPAL promoter containing cis-elements of the expression in xylem of Pinus taeda. The results suggested that AC elements of PtaPAL have multiple functions in the expression under the various developmental stages and stress conditions in the transgenic tobacco.

机译：我们从Peda taeda的PetaPAL中分离了苯丙氨酸解氨酶（PAL； EC 4.3.1.5）的基因的5'侧翼区域。为了研究PtaPAL启动子的组织特异性表达，使用表达PtaPAL启动子-GUS的转基因烟草进行GUS活性的组织化学测定。 PtaPAL启动子中的-897至-420区域在次生木质部中表现出高活性并响应弯曲应力。为了表征在苯丙烷生物合成中酶的启动子的顺式调节功能，我们研究了PtaPAL和4-香豆酸CoA连接酶Pta4CLα的嵌合启动子的活性。嵌合启动子显示出与Pta4CLα启动子相似的活性。电泳迁移率变动分析表明PtaPAL启动子的-897至–674含有taeda taeda木质部表达的顺式元件。结果表明，在转基因烟草的不同发育阶段和胁迫条件下，PtaPAL的AC元素在表达中具有多种功能。

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《Plant Cell Reports》 |2009年第9期|p.1309-1317|共9页
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Yuriko Osakabe; Keishi Osakabe; Vincent L. Chiang;
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1. Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum [J] . Osakabe Yuriko, Osakabe Keishi, Chiang Vincent L. Plant Cell Reports . 2009,第9期

机译：烟草中烟叶松（Pinus taeda）苯丙氨酸解氨酶基因启动子的组织特异性表达的表征
2. Isolation of 4-coumarate Co-A ligase gene promoter from loblolly pine (Pinus taeda) and characterization of tissue-specific activity in transgenic tobacco [J] . Osakabe Y, Osakabe K, Chiang VL Plant Physiology and Biochemistry . 2009,第11a12期

机译：从火炬松（Pinus taeda）中分离4-香豆酸Co-A连接酶基因启动子并鉴定转基因烟草中的组织特异性活性
3. Phenylalanine ammonia-lyase (PAL) from tobacco (Nicotiana tabacum): characterization of the four tobacco PAL genes and active heterotetrameric enzymes [J] . Angelika?I. Reichert, Richard?A. Dixon, Xian-Zhi He The biochemical journal . 2009,第2期

机译：烟草（Nicotiana tabacum）的苯丙氨酸氨裂合酶（PAL）：表征四种烟草PAL基因和活性异四聚酶
4. Phylogenomic Analysis of the Phenylalanine Ammonia Lyase Gene Family in Loblolly Pine (Pinus Taeda L.) [C] . Bagal Ujwal R., Leebens-Mack James H., Lorenz W. Walter, 2011 IEEE International Conference on Bioinformatics and Biomedicine . 2011

机译：火炬松（Pinus Taeda L.）中苯丙氨酸氨分解酶基因家族的系统生物学分析
5. Isolation and characterization of arginase promoters in loblolly pine (Pinus taeda L.) and Arabidopsis thaliana. [D] . Brownfield, Disa Lynn. 2007

机译：火炬松（Pinus taeda L.）和拟南芥中精氨酸酶启动子的分离和鉴定。
6. Assay and Electrophoresis of Superoxide Dismutase from Red Spruce (Picea rubens Sarg.) Loblolly Pine (Pinus taeda L.) and Scotch Pine (Pinus sylvestris L.) [O] . Norman E. Tandy, Richard T. Di Giulio, Curtis J. Richardson 1989

机译：红云杉（Picea rubens Sarg。）火炬松（Pinus taeda L.）和苏格兰松树（Pinus sylvestris L.）中超氧化物歧化酶的测定和电泳
7. Phenylalanine ammonia-lyase (PAL) from tobacco (Nicotiana tabacum): characterization of the four tobacco PAL genes and active heterotetrameric enzymes [O] . Reichert, Angelika I., He, Xian-Zhi, Dixon, R. A. 2009

机译：来自烟草（Nicotiana tabacum）的苯丙氨酸氨裂解酶（paL）：四种烟草paL基因和活性异四聚体酶的表征
8. Loblolly Pine: The Ecology and Culture of Loblolly Pine ('Pinus taeda L.') [R] . Schultz, R. P. 1997

机译：Loblolly pine：Loblolly pine（'pinus taeda L.'）的生态和文化

Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum

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