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首页> 外文期刊>Plant Cell Reports >Agrobacterium-mediated transformation of meadow fescue (Festuca pratensis Huds.)
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Agrobacterium-mediated transformation of meadow fescue (Festuca pratensis Huds.)

机译：农杆菌介导的草甸羊茅的转化（Festuca pratensis Huds。）

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摘要

Meadow fescue (Festuca pratensis Huds.) is an important cool-season forage grass in Europe and Asia. We developed a protocol for producing meadow fescue transgenic plants mediated by Agrobacterium tumefaciens transformation. Embryogenic calli derived from mature embryos were transformed with A. tumefaciens strain AGL1 carrying the binary vector pDM805, coding for the phosphinothricin acetyltransferase (bar) and β-glucuronidase (uidA) genes. Bialaphos was used as the selective agent throughout all phases of tissue culture. In total, 40 independent transgenic plants were recovered from 45 bialaphos-resistant callus lines and an average transformation efficiency of 2% was achieved. The time frame from infection of embryogenic calli with Agrobacterium to transferring the transgenic plants to the greenhouse was 18 weeks. In a study of 11 BASTA-resistant transgenic lines, the uidA gene was expressed in 82% of the transgenic lines. Southern blot analysis revealed that 82% of the tested lines integrated one or two copies of the uidA gene.
机译：草甸羊茅（Festuca pratensis Huds。）是欧洲和亚洲重要的凉季牧草。我们开发了一种生产根癌农杆菌转化介导的草甸羊茅转基因植物的方案。用携带二元载体pDM805的根癌农杆菌菌株AGL1转化衍生自成熟胚的胚发生愈伤组织，该二元载体编码膦丝菌素乙酰转移酶（bar）和β-葡萄糖醛酸苷酶（uidA）基因。在组织培养的所有阶段中，Bialaphos用作选择剂。总共从45个对双丙氨磷抗性的愈伤组织中回收了40株独立的转基因植物，平均转化效率达到2％。从土壤杆菌感染胚性愈伤组织到将转基因植物转移到温室的时间范围为18周。在对11个抗BASTA转基因株系的研究中，uidA基因在82％的转基因株系中表达。 Southern印迹分析显示82％的受试品系整合了一或两个拷贝的uidA基因。

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