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首页> 外文期刊>Plasma Science & Technology >A Mutant of Bacillus Subtilis with High-Producing Surfactin by Ion Beam Implantation
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A Mutant of Bacillus Subtilis with High-Producing Surfactin by Ion Beam Implantation

机译：离子束注入法制备高产表面肌动蛋白的枯草芽孢杆菌突变体

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摘要

In order to generate a mutant of Bacillus subtilis with enhanced surface activity through low energy nitrogen ion beam implantation, the effects of energy and dose of ions implanted were studied. The morphological changes in the bacteria were observed by scanning electron microscope (SEM). The optimum condition of ions implantation, 20 keV of energy and 2.6 x 10~(15)N~+/cm~2 in dose, was determined. A mutant, B.s-E-8 was obtained, whose surface activity of 50-fold and 100-fold diluted cell-free Landy medium was as 5.6-fold and 17.4-fold as the wild strain. The microbial growth and biosurfactant production of both the mutant and the wild strain were compared. After purified by ultrafiltration and SOURCE 15PHE, the biosurfactant was determined to be a complex of surfactin family through analysis of electrospray ionization mass spectrum (ESI/MS) and there was an interesting finding that after the ion beam implantation the intensities of the components were different from the wild type strain.
机译：为了通过低能氮离子束注入产生具有增强的表面活性的枯草芽孢杆菌突变体，研究了能量和离子注入剂量的影响。通过扫描电子显微镜（SEM）观察细菌的形态变化。确定了离子注入的最佳条件，能量为20 keV，剂量为2.6 x 10〜（15）N〜+ / cm〜2。获得突变体B.s-E-8，其50倍和100倍稀释的无细胞兰迪培养基的表面活性是野生菌株的5.6倍和17.4倍。比较了突变株和野生株的微生物生长和生物表面活性剂的产生。经过超滤和SOURCE 15PHE纯化后，通过电喷雾电离质谱（ESI / MS）分析，确定该生物表面活性剂是表面活性素家族的复合物，并且有一个有趣的发现，即离子束注入后组分的强度不同来自野生型菌株。

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