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首页> 外文期刊>Polar biology >Expression of amino acid transporter genes in developmental stages and adult tissues of Antarctic echinoderms
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Expression of amino acid transporter genes in developmental stages and adult tissues of Antarctic echinoderms

机译：氨基酸转运蛋白基因在南极棘皮动物发育阶段和成年组织中的表达

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摘要

Epithelial cells in the body wall of adult and developmental stages of marine invertebrates absorb dissolved organic material directly from seawater. Despite over a century of study, little is known about the molecular biological mechanisms responsible for this transport process. Previous studies on embryonic and larval Antarctic echinoderms show that amino acid uptake could provide an important supplement of metabolic substrates. In the present study, partial cDNA sequences of 11 putative amino acid transporter genes were isolated from six species of Antarctic echinoderms including the Antarctic sea stars Acodontaster hodgsoni, Diplasterias brucei, Odontaster meridionalis, Odontaster validus, and Perknaster fuscus, and the Antarctic sea urchin Sterechinus neumayeri. Conserved domains of cDNA-deduced amino acid sequences characterized these genes as being members of a family of amino acid transporters (solute carrier family 6). Expression of these genes was detected throughout embryonic and larval development of two species that have contrasting developmental modes (A. hodgsoni: lecithotrophic; O. meridionalis: planktotrophic). In all six species studied, the expression of amino acid transporter genes was detected in tube feet and digestive organs of adult animals, demonstrating that members of a single amino acid transporter gene family are expressed during the entire life history of a marine invertebrate. The identification of these genes is an important step toward developing a mechanistic understanding of amino acid transport capacities in Antarctic marine invertebrates.
机译：海洋无脊椎动物成年和发育阶段的体壁上皮细胞直接从海水中吸收溶解的有机物质。尽管进行了一个多世纪的研究，但对于导致这种转运过程的分子生物学机制知之甚少。以前对胚胎和幼体南极棘皮动物的研究表明，氨基酸的摄取可以提供代谢底物的重要补充。在本研究中，从包括南极海星Acodontaster hodgsoni，Diplasterias brucei，Odontaster meridionalis，Odontaster validus和Perknaster fuscus以及南极海胆立体动物在内的6种南极棘皮动物中分离出11个推定氨基酸转运蛋白基因的部分cDNA序列。 Neumayeri。 cDNA推导的氨基酸序列的保守结构域将这些基因表征为氨基酸转运蛋白家族的成员（溶质载体家族6）。这些基因的表达在具有相反发育模式的两个物种的整个胚胎和幼虫发育中都被检测到（A. hodgsoni：卵营养； O。meridionalis：浮游营养）。在所研究的所有六个物种中，在成年动物的管脚和消化器官中检测到了氨基酸转运蛋白基因的表达，表明单个氨基酸转运蛋白基因家族的成员在海洋无脊椎动物的整个生命历史中都表达。这些基因的鉴定是发展对南极海洋无脊椎动物中氨基酸转运能力的机械理解的重要步骤。

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  • 来源
    《Polar biology》 |2013年第9期|1257-1267|共11页
  • 作者

    Scott L. Applebaum; David W. Ginsburg; Charles S. Capron; Donal T. Manahan;

  • 作者单位

    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA;

    Environmental Studies Program, University of Southern California, Los Angeles, CA 90089-0036, USA;

    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA;

    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Antarctica; Echinoderms; Amino acid transporters; Gene families;

    机译：南极洲;棘皮动物;氨基酸转运蛋白;基因家族;

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