Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach

Kerstin Toebe; Bettina Meyer; Veronica Fuentes

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Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach

机译：使用分子方法检测胃中的浮游动物项目和虾磷虾幼虫的肠道含量

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The usefulness of a molecular approach based on polymerase chain reaction (PCR) was investigated to identify and quantify the feeding of larval krill on zoo-plankton organisms in the Lazarev Sea during winter in 2006. Different primers and probes of dominant copepod species (Oithona sp., Ctenocalanus citer, copepodid stages of Metridia gerlachei and Calanoides acutus), co-occurring with larval krill under sea ice during winter, were developed for quantitative PCR (qPCR) and their species specificity was tested on target and non-target species. The qPCR results showed that larval krill were exclusively feeding on Oithona sp. This result was confirmed by microscopic analysis of stomach and gut contents of larvae from the same stations.

机译：研究了一种基于聚合酶链反应（PCR）的分子方法在2006年冬季识别和量化拉扎列夫海的浮游生物对幼体磷虾的摄食的作用。优势co足类（Oithona sp。）的不同引物和探针（Ctenocalanus citer，Metridia gerlachei和Calanoides acutus的近足阶段），在冬季与海冰下的幼体磷虾共生，用于定量PCR（qPCR），并测试了它们对目标和非目标物种的特异性。 qPCR结果表明，幼体磷虾仅以Oithona sp。为食。通过显微镜分析同一站的幼虫的胃和肠内容物，证实了这一结果。

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《Polar biology》 |2010年第3期|407-414|共8页
作者
Kerstin Toebe; Bettina Meyer; Veronica Fuentes;
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作者单位

Scientific Division Polar Biological Oceanography, Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany;

Scientific Division Polar Biological Oceanography, Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany;

Institut de Ciencies del Mar (CSIC), Passeig Maritim de la Barcelona 37-49, Barcelona, Spain;

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正文语种 eng
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关键词
krill larvae; copepod prey; PCR; quantitative PCR;

机译：磷虾幼虫pe足类猎物PCR;定量PCR;

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专利

1. ANALYSIS OF THE STOMACH CONTENTS OF ANTARCTIC KRILL, EUPHAUSIA SUPERBA DANA [J] . 朱根海极地研究：英文版 . 1993,第002期

机译：南极KRILL胃含量分析，Euphausia Superba Dana
2. Molecular approach (PCR-DGGE) to diet analysis in young Antarctic krill Euphausia superba [J] . Daniel L. Martin, Robin M. Ross, Langdon B. Quetin, Marine ecology progress series . 2006,第Aug期

机译：分子方法（PCR-DGGE）对南极磷虾幼年磷虾的膳食进行分析
3. Zooplankton studies with special reference to krill Euphausia superba Dana from fishing area 58 of Indian Ocean sector in Southern Ocean. [J] . Rathod Vijayakumar Current Science: A Fortnightly Journal of Research . 2005,第4期

机译：浮游动物的研究特别参考了南洋印度洋扇贝捕捞区58的磷虾磷虾（Euphausia superba Dana）。
4. Effects of Cold Storage Environment Change on Antarctic Krill (Euphausia superba) Quality Center [C] . Shuai Chen, Hongliang Huang, Xueying Li, International Conference on Material Science, Energy and Environmental Engineering . 2017

机译：冷藏环境变化对南极磷虾（Euphausia Superba）优质中心的影响
5. On the ecology of larval krill, Euphausia superba, during winter: Krill-sea ice interactions. [D] . Frazer, Thomas K. 1995

机译：关于冬季磷虾幼虫的生态：磷虾与海冰的相互作用。
6. Composition and content analysis of fluoride in inorganic salts of the integument of Antarctic krill (Euphausia superba) [O] . Yuanhuai Peng, Wei Ji, Di Zhang, -1

机译：南极磷虾皮无机盐中氟化物的组成和含量分析
7. Protozoans as a food source for Antarctic krill, Euphausia superba: Complementary insights from stomach content, fatty acids, and stable isotopes [O] . Katrin Schmidt, Angus Atkinson, Klaus-jürgen Petzke, 2014

机译：原生动物作为南极磷虾（Euphausia superba）的食物来源：胃内容物，脂肪酸和稳定同位素的补充见解

Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach

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