• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Polar biology >Non-invasive Measurement Of Faecal Glucocorticoid Metabolites In Upland Geese Chloephaga Picta
【24h】

Non-invasive Measurement Of Faecal Glucocorticoid Metabolites In Upland Geese Chloephaga Picta

机译：陆鹅Chloephaga Picta的粪便糖皮质激素代谢产物的非侵入性测量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glucocorticoid (GC) hormones rise in response to stressors, including natural events including weather or predator presence, and human activities, such as hunting, scientific research or recreational visits. However, because blood sampling itself causes stress and is dangerous or even impossible in some wildlife species, feedback-free methods for GC determination are needed to assess stress in these animals. Faecal GC analyses have thus gained interest. Here, we validate a non-invasive method to estimate the physiological stress in the Upland goose Chloephaga picta. An adrenocorticotropin hormone (ACTH) challenge was conducted in captive adults (female and male), and droppings were collected before, during and after the experiment. Corticosterone metabolite (CM) secretion in response to the ACTH challenge was measured with several enzyme immunoassays (EIA) to find the most appropriate test. We used CM levels during the periods before and after the experiment as control data. An EIA for 11-oxoetiocholano-lone achieved the highest response to the ACTH challenge and also reflected a stress response to unfamiliar environ- ment. Furthermore, CM concentrations of dry samples were highly correlated with the corresponding non-dried (frozen) samples. The data suggest that this method is appropriate to measure the stress in Upland geese, and that samples can be stored either frozen or dry form.
机译：糖皮质激素（GC）激素会随着压力的增加而上升，包括自然事件（包括天气或掠食者的存在）以及人类活动（例如狩猎，科学研究或娱乐活动）。但是，由于采血本身会引起压力，并且在某些野生动植物物种中是危险的，甚至是不可能的，因此需要无反馈的GC测定方法来评估这些动物的压力。粪便GC分析因此引起了人们的兴趣。在这里，我们验证了一种非侵入性的方法来估计高地鹅Chloephaga picta的生理压力。在圈养的成年人（女性和男性）中进行肾上腺皮质激素激素（ACTH）攻击，并在实验之前，之中和之后收集粪便。用几种酶免疫测定法（EIA）测定了对ACTH激发应答的皮质酮代谢物（CM）分泌，以找到最合适的检测方法。我们在实验前后将CM水平用作对照数据。对11-氧代乙胆酸酮的环境影响评估对ACTH挑战的响应最高，也反映了对陌生环境的压力响应。此外，干燥样品的CM浓度与相应的非干燥（冷冻）样品高度相关。数据表明，该方法适用于测量高地鹅的压力，并且样品可以冷冻或干燥形式保存。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号