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首页> 外文期刊>Proceedings of the institution of mechanical engineers >Effects of nano-engineered surfaces on osteoblast adhesion, growth, differentiation, and apoptosis
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Effects of nano-engineered surfaces on osteoblast adhesion, growth, differentiation, and apoptosis

机译：纳米工程表面对成骨细胞粘附，生长，分化和凋亡的影响

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Modifying implant surfaces to improve their biocompatibility by enhancing osteoblast activation, growth, differentiation, and induction of greater bone formation with stronger attachments should result in improved outcomes for total joint replacement surgeries. This study tested the hypothesis that nano-structured surfaces, produced by the ion beam-assisted deposition method, enhance osteoblast adhesion, growth, differentiation, bone formation, and maturation. The ion beam-assisted deposition technique was employed to deposit zirconium oxide films on glass substrates. The effects of the ion beam-assisted deposition technique on cellular functions were investigated by comparing adhesion, proliferation, differentiation, and apoptosis of the human osteosarcoma cell line SAOS-2 on coated versus uncoated surfaces. Ion beam-assisted deposition nano-coatings enhanced initial cell adhesion assessed by the number of 4',6-diamidino-2-phe-nylindole-stained nuclei on zirconium oxide nano-coated surfaces compared to glass surfaces. This nano-modification also increased cell proliferation as measured by mitochondrial dehydrogenase activity. Moreover, the ion beam-assisted deposition technique improved cell differentiation as determined by the formation of mineralized bone nodules and by the rate of calcium deposition, both of which are in vitro indicators of the successful bone formation. However, programmed cell death assessed by Annexin V staining and flow cytometry was not statistically significantly different between nano-surfaces and glass surfaces. Overall, the results indicate that nano-crystalline zirconium oxide surfaces produced by the ion beam-assisted deposition technique are superior to uncoated surfaces in supporting bone cell adhesion, proliferation, and differentiation. Thus, surface properties altered by the ion beam-assisted deposition technique enhanced bone formation and may increase the biocompatibility of bone cell-associated surfaces.
机译：通过增强成骨细胞的活化，生长，分化和诱导更大的骨形成以及更强的附着力来修饰植入物表面，从而改善其生物相容性，这应该会改善全关节置换手术的结果。这项研究检验了以下假设：通过离子束辅助沉积方法产生的纳米结构表面可增强成骨细胞的附着力，生长，分化，骨形成和成熟。采用离子束辅助沉积技术在玻璃基板上沉积氧化锆膜。通过比较人骨肉瘤细胞系SAOS-2在有涂层和无涂层表面上的粘附，增殖，分化和凋亡，研究了离子束辅助沉积技术对细胞功能的影响。与玻璃表面相比，通过氧化锆纳米涂层表面上的4'，6-二mid基-2-苯并吲哚吲哚染色的核数评估，离子束辅助沉积纳米涂层增强了初始细胞粘附性。通过线粒体脱氢酶活性测定，这种纳米修饰还增加了细胞增殖。此外，离子束辅助沉积技术改善了细胞分化，这取决于矿化的骨结节的形成和钙沉积的速率，这两者都是成功骨形成的体外指标。但是，通过膜联蛋白V染色和流式细胞仪评估的程序性细胞死亡在纳米表面和玻璃表面之间没有统计学差异。总体而言，结果表明，通过离子束辅助沉积技术生产的纳米晶氧化锆表面在支持骨细胞粘附，增殖和分化方面优于未涂覆表面。因此，通过离子束辅助沉积技术改变的表面性质增强了骨的形成，并可能增加了与骨细胞相关的表面的生物相容性。

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