...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Expression cloning of a cDNA encoding a fish prolactin receptor.
【24h】

Expression cloning of a cDNA encoding a fish prolactin receptor.

机译：编码鱼催乳激素受体的cDNA的表达克隆。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By using an expression cloning strategy, we isolated a single positive clone encoding a tilapia prolactin (PRL) receptor. Tilapia PRL188 was used to screen a freshwater tilapia kidney expression library transfected in COS cells. The tilapia PRL receptor is a mature protein of 606 amino acids. The extracellular domain is devoid of the tandem repeat units present in birds and has two pairs of cysteine residues, a Trp-Ser-Xaa-Trp-Ser motif, and two potential N-glycosylation sites. The cytoplasmic domain contains 372 amino acids, including box 1, a sequence previously shown to be important for signal transduction in mammalian species. Thus, the general structure is similar to the long form of mammalian PRL receptors; however, amino acid comparisons reveal a rather low identity (approximately 37%). Northern blot analysis shows the existence of a single transcript in osmoregulatory tissues and reproductive organs. This localization is in agreement with known functions of PRL in teleosts.
机译：通过使用表达克隆策略，我们分离了一个编码罗非鱼催乳素（PRL）受体的单个阳性克隆。罗非鱼PRL188用于筛选在COS细胞中转染的淡水罗非鱼肾脏表达文库。罗非鱼PRL受体是606个氨基酸的成熟蛋白。细胞外结构域没有禽类中存在的串联重复单元，并且具有两对半胱氨酸残基，一个Trp-Ser-Xaa-Trp-Ser基序和两个潜在的N-糖基化位点。胞质结构域包含372个氨基酸，包括框1，该序列先前显示对哺乳动物物种的信号转导很重要。因此，总体结构类似于哺乳动物PRL受体的长形式。但是，氨基酸比较显示出较低的同一性（约37％）。 Northern印迹分析表明在渗透调节组织和生殖器官中存在单个转录物。这种定位与硬骨鱼类中PRL的已知功能一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号