...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >ADENOVIRAL E1B-55KDA PROTEIN INHIBITS YEAST MRNA EXPORT AND PERTURBS NUCLEAR STRUCTURE
【24h】

ADENOVIRAL E1B-55KDA PROTEIN INHIBITS YEAST MRNA EXPORT AND PERTURBS NUCLEAR STRUCTURE

机译：腺病毒E1B-55KDA蛋白质抑制酵母MRNA出口并扰乱核结构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mechanisms of export of RNA from the nucleus are poorly understood; however, several viral proteins modulate nucleocytoplasmic transport of mRNA. Among these are the adenoviral proteins E1B-55kDa and E4-34kDa. Late in infection, these proteins inhibit export of host transcripts and promote export of viral mRNA. To investigate the mechanism by which these proteins act, we have expressed them in Saccharomyces cerevisiae. Overexpression of either or both proteins has no obvious effect on cell. growth. By contrast, overexpression of E1B-55kDa bearing a nuclear localization signal (NLS) dramatically inhibits cell growth. In this situation, the NLS-E1B-55kDa protein is localized to the nuclear periphery, fibrous material is seen in the nucleoplasm, and poly(A)(+) RNA accumulates in the nucleus, Simultaneous overexpression of E4-34kDa bearing or lacking an NLS does not modify these effects. We discuss the mechanisms of selective mRNA transport. [References: 47]
机译：从细胞核中输出RNA的机制了解甚少；但是，几种病毒蛋白调节mRNA的核质转运。其中包括腺病毒蛋白E1B-55kDa和E4-34kDa。在感染后期，这些蛋白质抑制宿主转录本的输出并促进病毒mRNA的输出。为了研究这些蛋白质起作用的机制，我们已经在酿酒酵母中表达了它们。一种或两种蛋白质的过表达对细胞没有明显影响。增长。相比之下，带有核定位信号（NLS）的E1B-55kDa的过表达会显着抑制细胞生长。在这种情况下，NLS-E1B-55kDa蛋白位于核外围，在核质中可见纤维物质，poly（A）（+）RNA积聚在细胞核中，同时E4-34kDa携带或缺乏E4-34kDa的过表达NLS不会修改这些效果。我们讨论选择性mRNA运输的机制。 [参考：47]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号