...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >GA-binding protein factors, in concert with the coactivator CREB binding protein/p300, control the induction of the interleukin 16 promoter in T lymphocytes
【24h】

GA-binding protein factors, in concert with the coactivator CREB binding protein/p300, control the induction of the interleukin 16 promoter in T lymphocytes

机译：GA结合蛋白因子与共激活因子CREB结合蛋白/ p300共同控制T淋巴细胞中白介素16启动子的诱导

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Interleukin 16 (IL-16) is a chemotactic cy- tokine that binds to the CD4 receptor and affects the activa- tion of T cells and replication of HIV. It is expressed as a large 67-kDa precursor protein (pro-IL-16) in lymphocytes, mac- rophages, and mast cells, as well as in airway epithelial cells from asthmatics after challenge with allergen. This pro-IL-16 is subsequently processed to the mature cytokine of 13 kDa. To study the expression of IL-16 at the transcriptional level, we cloned the human chromosomal IL-16 gene and analyzed its promoter. The human IL-16 gene consists of seven exons and six introns. The 5' sequences up to nucleotide -120 of the human and murine IL-16 genes share >84 sequence homol- ogy and harbor promoter elements for constitutive and in- ducible transcription in T cells. Although both promoters lack any TATA box, they contain two CAAT box-like motifs and three binding sites of GA-binding protein (GABP) transcrip- tion factors. Two of these motifs are part of a highly conserved and inducible dyad symmetry element shown previously to control a remote IL-2 enhancer and the CD18 promoter. In concert with the coactivator CREB binding protein/p300, which interacts with GABP#alpha#, the binding of GABP#alpha# and -#beta# to the dyad symmetry element controls the induction of IL-16 promoter in T cells. Supplementing the data on the processing of pro-IL-16, our results indicate the complexity of IL-16 expression, which is tightly controlled at the transcriptional and posttranslational levels in T lymphocytes.
机译：白细胞介素16（IL-16）是一种趋化性细胞因子，与CD4受体结合并影响T细胞的活化和HIV的复制。在过敏原激发后，它在淋巴细胞，巨噬细胞和肥大细胞以及哮喘患者的气道上皮细胞中以67kDa的大型前体蛋白（pro-IL-16）表达。随后将该原IL-16加工成13kDa的成熟细胞因子。为了研究IL-16在转录水平上的表达，我们克隆了人类染色体IL-16基因并分析了其启动子。人IL-16基因由七个外显子和六个内含子组成。人类和鼠类IL-16基因的核苷酸-120之前的5'序列具有> 84的序列同源性，并具有启动子元件，可在T细胞中进行组成型和诱导型转录。尽管两个启动子都没有任何TATA框，但它们包含两个CAAT框样基序和三个GA结合蛋白（GABP）转录因子的结合位点。这些基序中的两个是先前显示的控制远程IL-2增强子和CD18启动子的高度保守且可诱导的dyad对称元件的一部分。与与GABP＃alpha＃相互作用的共激活因子CREB结合蛋白/ p300协同作用，GABP＃alpha＃和-＃beta＃与dyad对称元件的结合控制了T细胞中IL-16启动子的诱导。补充有关pro-IL-16加工的数据，我们的结果表明IL-16表达的复杂性，在T淋巴细胞的转录和翻译后水平上受到严格控制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号