Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis

Stefan W. Schneider; Kumudesh C. Sritharan; John P. Geibel

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Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis

机译：原子力显微镜成像的活腺泡细胞的表面动力学：胞吐作用涉及的质膜结构的鉴定

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The dynamics at the plasma membrane result- ng from secretory vesicle docking and fusion and compensatory endocytosis has been difficult to observe in living cells primarily due to limited resolution at the light microscopic level. Using the atomic force microscope, we have been able to image and record changes in plasma membrane structure at ultrahigh resolution after stimulation of secretion from isolated pancreatic acinar cells. "Pits" measuring 500-2000 nm and containing 3-20 de- ressions measuring 100-180 nm in diameter were observed only at the apical region of acinar cells.

机译：由于在光学显微镜下分辨率有限，很难在活细胞中观察到由分泌性囊泡对接和融合以及代偿性内吞作用引起的质膜动力学。使用原子力显微镜，我们能够在刺激并刺激胰腺胰腺腺泡细胞分泌后以超高分辨率记录并记录质膜结构的变化。仅在腺泡细胞的顶端区域观察到尺寸为500-2000 nm且包含3-20个直径为100-180 nm的凹陷的“凹坑”。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第1期|p.316-321|共6页
作者
Stefan W. Schneider; Kumudesh C. Sritharan; John P. Geibel;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Atomic force microscopy of microvillous cell surface dynamics at fixed and living alveolar type II cells [J] . Hecht E., Usmani S.M., Albrecht S., Analytical and bioanalytical chemistry . 2011,第7期

机译：原子力显微镜观察固定和活着的II型肺泡细胞微绒毛细胞表面动力学
2. Atomic force microscopy of microvillous cell surface dynamics at fixed and living alveolar type II cells [J] . Elena Hecht, Shariq M. Usmani, Susanne Albrecht, Analytical and Bioanalytical Chemistry . 2011,第7期

机译：原子力显微镜观察固定和活着的II型肺泡细胞微绒毛细胞表面动力学
3. Specific detection of glycans on a plasma membrane of living cells with atomic force microscopy [J] . Lekka M, Laidler P, Labedz M, Chemistry & biology . 2006,第5期

机译：原子力显微镜特异性检测活细胞质膜上的聚糖
4. Local Plasma Membrane Permeabilization of Living Cells by Nanosecond Electric Pulses Using Atomic Force Microscopy [C] . Gary Thompson, Jason A Payne, Caleb C. Roth, Conference on nanoscale imaging, sensing, and actuation for biomedical applications VIII . 2011

机译：纳秒电脉冲使用原子力显微镜对活细胞的局部血浆膜通透性
5. The Hsp70 cochaperone cysteine string protein (CSP) interacts with acinar cell membrane fusion machinery and regulates exocytosis. [D] . Weng, Ning. 2006

机译：Hsp70陪伴酮半胱氨酸串蛋白（CSP）与腺泡细胞膜融合机制相互作用并调节胞吐作用。
6. Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis [O] . Stefan W. Schneider, Kumudesh C. Sritharan, John P. Geibel, 1997

机译：原子力成像的活腺泡细胞的表面动力学显微镜：鉴定所涉及的质膜结构胞吐
7. Imaging of the surface of living cells by low-force contact-mode atomic force microscopy. [O] . Le Grimellec, C, Lesniewska, E, Giocondi, M C, 1998

机译：低力接触模式原子力显微镜对活细胞表面的成像。

Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis

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