High-resolution functional mapping of a cloned gene by genetic footprinting

Ila R. Singh; Rachel Adams Crowley; Patrick O. Brown

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High-resolution functional mapping of a cloned gene by genetic footprinting

机译：通过基因足迹对克隆基因进行高分辨率功能定位

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摘要

We describe an efficient method for intro- ducing and analyzing a comprehensive set of mutations in a cloned gene to map its functional organization. The technique, genetic footprinting, uses a retroviral integrase to generate a comprehensive library of mutants, each of which bears a single insertion of a defined oligonucleotide at a random position in the gene of interest. This mutant library is selected for gene function en masse. DNA samples are isolated from the library both before and after selection, and the mutations represented in each sample are then analyzed.

机译：我们描述了一种有效的方法，用于在克隆的基因中引入和分析一组全面的突变，以绘制其功能组织图。遗传足迹技术是一种利用逆转录病毒整合酶来产生突变体的综合库，每个突变体在目标基因的随机位置上都带有一个插入的已定义寡核苷酸。选择该突变体文库以实现基因功能。在选择之前和之后都从文库中分离DNA样品，然后分析每个样品中代表的突变。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第4期|p.1304-1309|共6页
作者
Ila R. Singh; Rachel Adams Crowley; Patrick O. Brown;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Genetic mapping, cloning, and functional characterization of the BnaX.VTE4 gene encoding a d-tocopherol methyltransferase from oilseed rape [J] . Endrigkeit Jessica, Wang Xingxing, Cai Daguang, Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics . 2009,第3期

机译：油菜油菜d-生育酚甲基转移酶BnaX.VTE4基因的遗传作图，克隆及功能鉴定
2. Genetic mapping, cloning, and functional characterization of the BnaX.VTE4 gene encoding a γ-tocopherol methyltransferase from oilseed rape [J] . Jessica Endrigkeit, Xingxing Wang, Daguang Cai, TAG Theoretical and Applied Genetics . 2009,第3期

机译：油菜油菜编码γ-生育酚甲基转移酶的BnaX.VTE4基因的遗传定位，克隆和功能表征
3. Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome. [J] . McCarthy L, Hunter K, Schalkwyk L, Proceedings of the National Academy of Sciences of the United States of America . 1995,第12期

机译：高效的小鼠散布的重复序列PCR产物的高分辨率遗传图谱，朝向小鼠基因组的综合遗传图谱和物理图谱。
4. Map based cloning of a receptor kinase gene (Nork) by genetic mapping of a mutation (nn_1) conditioning non-nodulating phenotype in the tetraploid alfalfa mutant MN-1008 [C] . G. B. Kiss, S. Mihacea, Z. Kevei, International Congress on Nitrogen Fixation . 2002

机译：通过突变（NN_1）调节在四倍体Alfalfa突变体MN-1008中的突变映射（NN_1）条件的遗传映射来克隆受体激酶基因（Nork）的克隆
5. Development and applications of genetic footprinting, a method for high-resolution functional mapping of cloned genes. [D] . Crowley, Rachel Adams. 1999

机译：遗传足迹的开发和应用，一种用于克隆基因的高分辨率功能作图的方法。
6. High-resolution functional mapping of a cloned gene by genetic footprinting [O] . Ila R. Singh, Rachel Adams Crowley, Patrick O. Brown 1997

机译：遗传足迹法对克隆基因的高分辨率功能作图
7. Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome. [O] . McCarthy, L, Hunter, K, Schalkwyk, L, 1995

机译：高效高效的小鼠散布的重复序列PCR产物的高分辨率遗传图谱，朝着小鼠基因组的综合遗传图谱和物理图谱的方向发展。
8. Genetic Alterations in Familial Breast Cancer: Mapping and Cloning Genes Other Than BRCAl [R] . King, M. C. , Winkelstein, W. 1998

机译：家族性乳腺癌的遗传改变：定位和克隆BRCa1以外的基因

High-resolution functional mapping of a cloned gene by genetic footprinting

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