The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis

Virginie Durbecq; Christianne Legrain; Martine Roovers

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The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis

机译：嗜热古细菌激烈热球菌的氨基甲酸酯激酶样氨基甲酰磷酸合成酶，是氨基甲酰磷酸生物合成过程中缺失的环节

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Microbial carbamoyl phosphate synthetases (CPS) use glutamine as nitrogen donor and are composed of two subunits (or domains), one exhibiting glutaminase activ- ity, the other able to synthesize carbamoyl phosphate (CP) from bicarbonate, ATP, and ammonia. The pseudodimeric organization of this synthetase suggested that it has evolved by duplication of a smaller kinase, possibly a carbamate kinase (CK). In contrast to other prokaryotes the hyperthermophilic archaeon Pyrococcus furiosus was found to synthesize CP by using ammonia and not glutamine. We have purified the cognate enzyme and found it to be a dimer of two identical subunits of Mr 32,000. Its thermostability is considerable, 50/100 activity being retained after 1 h at 100 deg C or 3 h at 95 deg C.

机译：微生物氨基甲酸酯磷酸酯合成酶（CPS）使用谷氨酰胺作为氮供体，由两个亚基（或域）组成，一个具有谷氨酰胺酶活性，另一个能够从碳酸氢盐，ATP和氨中合成氨基甲酸酯磷酸酯（CP）。该合成酶的假二聚体组织表明，它是通过复制较小的激酶（可能是氨基甲酸酯激酶（CK））而进化而来的。与其他原核生物相反，发现超嗜热古生火球菌通过使用氨而不是谷氨酰胺合成CP。我们已经纯化了关联酶，发现它是Mr 32,000的两个相同亚基的二聚体。它的热稳定性非常好，在100摄氏度1小时后或在95摄氏度3小时后仍保持50/100活性。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第24期|p.12803-12808|共6页
作者
Virginie Durbecq; Christianne Legrain; Martine Roovers;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. The 1.5 A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insig [J] . Ramon Maiques-S, Marina A, Uriarte M, Journal of Molecular Biology . 2000,第2期

机译：来自超嗜热古细菌激烈球菌的氨基甲酸酯激酶样氨基甲酰磷酸合成酶的1.5 A分辨率晶体结构与ADP结合，证实该热稳定酶是氨基甲酸酯激酶，可提供insig
2. Carbamate kinase can replace in vivo carbamoyl phosphate synthetase. Implications for the evolution of carbamoyl phosphate biosynthesis [J] . Alcaacute, ntara Cristina, Cervera Javier, FEBS Letters . 2000,第3期

机译：氨基甲酸酯激酶可以代替体内氨基甲酰磷酸合成酶。对氨基甲酰磷酸生物合成进化的影响
3. Ammonia-dependent synthesis and metabolic channelling of carbamoyl phosphate in the hyperthermophilic archaeon Pyrococcus furiosus [J] . Christianne Legrain, Marc Demarez, Nicolas Glansdorff, Microbiology . 1995,第5期

机译：氨基依赖性合成和氨基甲磷酸盐磷酸盐磷酸盐磷酸盐磷酸盐的代谢窜
4. Detection of Intermediates in the Carbamoyl Phosphate Synthetase Reaction [C] . Frank M. Raushel, Leisha S. Mullins, Grant Gibson Steenbock Symposium on Enzymatic Mechanisms . 1999

机译：检测氨基酰磷酸盐合成酶反应中的中间体
5. Characterization of carbamoyl phosphate synthetase 1 palmitoylation and development of a chemical biology approach to detect and identify new palmitoylated proteins. [D] . Corvi, Maria Martha. 2006

机译：氨基甲酰基磷酸合成酶1棕榈酰化的表征和化学生物学方法的发展，以检测和鉴定新的棕榈酰化蛋白。
6. The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus a missing link in the evolution of carbamoyl phosphate biosynthesis [O] . Virginie Durbecq, Christianne Legrain, Martine Roovers, 1997

机译：嗜热古细菌激烈热球菌的氨基甲酸酯激酶样氨基甲酸酯磷酸合成酶是氨基甲酸酯磷酸生物合成过程中缺失的环节
7. The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis [O] . Durbecq, Virginie, Legrain, Christianne, Roovers, Martine, 1997

机译：嗜热古细菌激烈热球菌的氨基甲酸酯激酶样氨基甲酰磷酸合成酶，是氨基甲酰磷酸生物合成过程中缺失的环节

The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis

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