An interaction between DNA ligase I and proliferating cell nuclear antigen: Implications for Okazaki fragment synthesis and joining

David S. Levin; Wei Bai; Nina Yao

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An interaction between DNA ligase I and proliferating cell nuclear antigen: Implications for Okazaki fragment synthesis and joining

机译：DNA连接酶I与增殖细胞核抗原之间的相互作用：对冈崎片段合成和连接的影响

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Although three human genes encoding DNA ligases have been isolated, the molecular mechanisms by which these gene products specifically participate in different DNA transactions are not well understood. In this study, fractionation of a HeLa nuclear extract by DNA ligase I affinity chromatography resulted in the specific retention of a replication protein, proliferating cell nuclear antigen (PCNA), by the affinity resin. Subsequent experiments dem- onstrated that DNA ligase I and PCNA interact directly via the amino-terminal 118 aa of DNA ligase I, the same region of DNA ligase I that is required for localization of this enzyme at replication foci during S phase.

机译：尽管已分离出三个编码DNA连接酶的人类基因，但人们对这些基因产物特异性参与不同DNA交易的分子机制尚不十分了解。在这项研究中，通过DNA连接酶I亲和层析对HeLa核提取物进行分级分离，导致亲和树脂特异性保留复制蛋白（增殖细胞核抗原（PCNA））。随后的实验表明，DNA连接酶I和PCNA通过DNA连接酶I的氨基末端118aa直接相互作用，DNA连接酶I的同一区域是该酶在S期定位于复制位点所需的相同区域。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第24期|p.12863-12868|共6页
作者
David S. Levin; Wei Bai; Nina Yao;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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专利

1. Interaction between PCNA and DNA ligase I is critical for joining of Okazaki fragments and long-patch base-excision repair [J] . Levin DS., Motycka TA., Matsumoto Y., Current Biology: CB . 2000,第15期

机译：PCNA和DNA连接酶I之间的相互作用对于Okazaki片段的连接和长片段碱基切除修复至关重要
2. A single amino acid substitution in the DNA-binding domain of Aeropyrum pernix DNA ligase impairs its interaction with proliferating cell nuclear antigen [J] . Shinichi Kiyonari, Toru Kamigochi, Yoshizumi Ishino Extremophiles . 2007,第5期

机译：Aeropyrum pernix DNA连接酶的DNA结合结构域中的单个氨基酸取代会削弱其与增殖细胞核抗原的相互作用
3. C-terminal flap endonuclease (rad27) mutations: lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. [J] . Karanja KK, Livingston DM Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2009,第1期

机译：C末端皮瓣内切核酸酶（rad27）突变：与DNA连接酶I突变（cdc9-p）的致命相互作用，并通过酿酒酵母中细胞核抗原（POL30）的增殖抑制。
4. Low Power Laser Irradiation Stimulates Cell Proliferation via Proliferating Cell Nuclear Antigen and Ki-67 Expression during Tissue Repair [C] . Vijendra Prabhu, Bola Sadashiva Satish Rao, Krishna Kishore Mahato Mechanisms for low-light therapy X . 2015

机译：低功率激光辐照在组织修复过程中通过增殖细胞核抗原和Ki-67表达刺激细胞增殖。
5. C-terminal flap endonuclease (rad27) mutations: Lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. [D] . Karanja, Kenneth Kimani. 2009

机译：C末端皮瓣内切核酸酶（rad27）突变：与酿酒酵母中DNA连接酶I突变（cdc9-p）的致命相互作用以及通过增殖细胞核抗原（POL30）的抑制作用。
6. An interaction between DNA ligase I and proliferating cell nuclear antigen: Implications for Okazaki fragment synthesis and joining [O] . David S. Levin, Wei Bai, Nina Yao, 1997

机译：DNA连接酶I与增殖细胞核抗原之间的相互作用：冈崎片段合成和连接的含义
7. A conserved interaction between the replicative clamp loader and DNA ligase in eukaryotes: implications for Okazaki fragment joining [O] . David S. Levin, Sangeetha Vijayakumar, Xiuping Liu, 2013

机译：真核生物中复制钳装载器和DNA连接酶之间的保守相互作用：对冈崎片段连接的影响。

An interaction between DNA ligase I and proliferating cell nuclear antigen: Implications for Okazaki fragment synthesis and joining

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