Yeast microarrays for genome wide parallel genetic and gene expression analysis

Deval A. Lashkari; Joseph L. DeRisi; John H. McCusker

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Yeast microarrays for genome wide parallel genetic and gene expression analysis

机译：酵母微阵列，用于全基因组并行遗传和基因表达分析

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We have developed high-density DNA mi- croarrays of yeast ORFs. These microarrays can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for se- quence polymorphisms. Automated scripts retrieved sequence information from public databases to locate predicted ORFs and select appropriate primers for amplification. The primers were used to amplify yeast ORFs in 96-well plates, and the resulting products were arrayed using an automated micro arraying device. Arrays containing up to 2,479 yeast ORFs were printed on a single slide. The hybridization of fluores- cently labeled samples to the array were detected and quan- titated with a laser confocal scanning microscope.

机译：我们已经开发了酵母ORF的高密度DNA微阵列。这些微阵列可以监测与ORF的杂交，以进行定量差异基因表达分析和序列多态性筛选等应用。自动化脚本从公共数据库检索序列信息，以定位预测的ORF并选择合适的引物进行扩增。引物用于在96孔板中扩增酵母ORF，并使用自动微阵列设备对所得产物进行阵列。包含多达2,479个酵母ORF的阵列被印在一张幻灯片上。检测了荧光标记样品与阵列的杂交，并用激光共聚焦扫描显微镜定量。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第24期|p.13057-13062|共6页
作者
Deval A. Lashkari; Joseph L. DeRisi; John H. McCusker;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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6. Yeast microarrays for genome wide parallel genetic and gene expression analysis [O] . Deval A. Lashkari, Joseph L. DeRisi, John H. McCusker, 1997

机译：用于全基因组平行遗传和基因表达分析的酵母微阵列
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机译：并行人类基因组分析：基于微阵列的1000个基因表达监测。

Yeast microarrays for genome wide parallel genetic and gene expression analysis

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