Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells

John R. Zupan; Vitaly Citovsky; Patricia Zambryski

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Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells

机译：农杆菌VirE2蛋白介导植物细胞中单链DNA的核吸收

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Agrobacterium genetically transforms plant cells by transferring a single-stranded DNA (ssDNA) copy of the transferred DNA (T-DNA) element, the T-strand, in a complex with Agrobacterium proteins VirD2, bound to the 5' end, and VirE2. VirE2 binds single-stranded nucleic acid cooperatively, fully coating the T-strand, and the protein localizes to the plant cell nucleus when transiently expressed.

机译：农杆菌通过与被结合到5'端的农杆菌蛋白VirD2和VirE2形成复合体，通过转移被转移的DNA（T-DNA）元素的单链DNA（ssDNA）拷贝T链来遗传转化植物细胞。 VirE2协同结合单链核酸，完全覆盖T链，并且蛋白质在瞬时表达时定位于植物细胞核。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第6期|p.2392-2397|共6页
作者
John R. Zupan; Vitaly Citovsky; Patricia Zambryski;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. VirE1 protein mediates export of the single-stranded DNA-binding protein VirE2 from Agrobacterium tumefaciens into plant cells. [J] . C Sundberg, L Meek, K Carroll, Journal of bacteriology . 1996,第4期

机译：VirE1蛋白介导单链DNA结合蛋白VirE2从根癌农杆菌输出到植物细胞中。
2. Agrobacterium rhizogenes GALLS Protein Substitutes for Agrobacterium tumefaciens Single-Stranded DNA-Binding Protein VirE2 [J] . Larry D. Hodges, Josh Cuperus, Walt Ream Journal of bacteriology . 2004,第10期

机译：发根农杆菌GALLS根癌农杆菌单链DNA结合蛋白VirE2的蛋白质替代物
3. Mutagenesis of the Agrobacterium VirE2 Single-Stranded DNA-Binding Protein Identifies Regions Required for Self-Association and Interaction with VirE1 and a Permissive Site for Hybrid Protein Construction [J] . Xue-Rong Zhou, Peter J. Christie Journal of bacteriology . 1999,第14期

机译：农杆菌VirE2单链DNA结合蛋白的诱变识别自我关联和与VirE1相互作用和杂合蛋白构建的允许位点所需的区域
4. Reducing Batch-to-Batch Variability of Agrobacterium-Mediated Transient Protein Expression In Plant Tissue Culture [C] . Jeffrey S. Larsen, Wayne R. Curtis American Institute of Chemical Engineers Annual Meeting . 2008

机译：降低农杆菌介导的植物组织培养中介导的瞬态蛋白表达的批量变异性
5. Isolation and characterization of acDNA encoding a plant phosphatase implicated in nuclear import of the Agrobacterium VirD2 protein. [D] . Tao, Yumin. 1998

机译：编码与农杆菌VirD2蛋白核输入有关的植物磷酸酶的cDNA的分离和鉴定。
6. Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells. [O] . J R Zupan, V Citovsky, P Zambryski 1996

机译：农杆菌VirE2蛋白介导植物细胞中单链DNA的核吸收。
7. Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells. [O] . Zupan, J R, Citovsky, V, Zambryski, P 1996

机译：农杆菌VirE2蛋白介导植物细胞中单链DNA的核吸收。
8. Analysis of proteins essential for Agrobacterium mediated DNA transfer to plant cells. (Progress report). [R] . 1989

机译：分析农杆菌介导的DNa转移到植物细胞必需的蛋白质。（进度报告）。

Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells

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