Analysis of metabolic pathways by the growth of cells in the presence of organic solvents

Henry E. Spinnler; Christian Ginies; Jeffrey A. Khan

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Analysis of metabolic pathways by the growth of cells in the presence of organic solvents

机译：在有机溶剂存在下通过细胞生长分析代谢途径

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A new approach to the analysis of metabolic pathways involving poorly water-soluble intermediates is pro- posed. It relies upon the ability of the hydrophobic interme- diates formed by a sequence of intracellular reactions to cross the membrane(s) and partition between aqueous and organic phases, when cells are incubated in the presence of a nonpolar and nontoxic organic solvent. As a result of this thermody- namically driven efflux of the formed intermediates from the cell, they accumulate in the organic medium in sufficient quantities for GC-MS analysis and identification.

机译：提出了一种新的方法来分析涉及水溶性差的中间体的代谢途径。当细胞在非极性和无毒有机溶剂存在下孵育时，它依赖于一系列细胞内反应形成的疏水性中间体穿过膜并在水相和有机相之间分配的能力。由于热动力学驱动了形成的中间体从池中流出，因此中间体在有机介质中积累的量足以进行GC-MS分析和鉴定。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第8期|p.3373-3376|共4页
作者
Henry E. Spinnler; Christian Ginies; Jeffrey A. Khan;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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Analysis of metabolic pathways by the growth of cells in the presence of organic solvents

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