...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cellular solid-state nuclear magnetic resonance spectroscopy
【24h】

Cellular solid-state nuclear magnetic resonance spectroscopy

机译：细胞固态核磁共振波谱

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Decrypting the structure, function, and molecular interactions of complex molecular machines in their cellular context and at atomic resolution is of prime importance for understanding fundamental physiological processes. Nuclear magnetic resonance is a well-established imaging method that can visualize cellular entities at the micrometer scale and can be used to obtain 3D atomic structures under in vitro conditions. Here, we introduce a solid-state NMR approach that provides atomic level insights into cell-associated molecular components. By combining dedicated protein production and labeling schemes with tailored solid-state NMR pulse methods, we obtained structural information of a recombinant integral memb'rane protein and the major endogenous molecular components in a bacterial environment. Our approach permits studying entire cellular compartments as well as cell-associated proteins at the same time and at atomic resolution.
机译：解密复杂分子机器在其细胞内和原子分辨率下的结构，功能和分子相互作用对于理解基本的生理过程至关重要。核磁共振是一种成熟的成像方法，可以在微米级可视化细胞实体，并可以用于在体外条件下获得3D原子结构。在这里，我们介绍了一种固态NMR方法，该方法可提供原子级的洞察到与细胞相关的分子成分。通过将专用的蛋白质生产和标记方案与量身定制的固态NMR脉冲方法相结合，我们获得了细菌环境中重组整体膜蛋白和主要内源性分子成分的结构信息。我们的方法允许在原子分辨率下同时研究整个细胞区室以及与细胞相关的蛋白质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号