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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Feline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats
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Feline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats

机译：猫性杯状病毒，一种以前未曾描述过的副粘病毒，与家猫的肾小管间质性肾炎有关

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摘要

We describe the discovery and isolation of a paramyxovirus, feline morbillivirus (FmoPV), from domestic cat (Felis catus). FmoPV RNA was detected in 56 (12.3%) of 457 stray cats (53 urine, four rectal swabs, and one blood sample) by RT-PCR. Complete genome sequencing of three FmoPV strains showed genome sizes of 16,050 bases, the largest among morbilliviruses, because of unusually long 5' trailer sequences of 400 nt. FmoPV possesses identical gene contents (3-N-P/V/C-M-F-H-L-5) and is phylogenetically clustered with other morbilliviruses. IgG against FmoPV N protein was positive in 49 sera (76.7%) of 56 RT-PCR-positive cats, but 78 (19.4%) of 401 RTPCR-negative cats (P < 0.0001) by Western blot. FmoPV was isolated from CRFK feline kidney cells, causing cytopathic effects with cell rounding, detachment, lysis, and syncytia formation. FmoPV could also replicate in subsequent passages in primate Vero E6 cells. Infected cell lines exhibited finely granular and diffuse cytoplasmic fluorescence on immunostaining for FmoPV N protein. Electron microscopy showed enveloped virus with typical "herringbone" appearance of helical N in paramyxoviruses. Histological examination of necropsy tissues in two FmoPV-positive cats revealed interstitial inflammatory infiltrate and tubular degenerationecrosis in kidneys, with decreased cauxin expression in degenerated tubular epithelial cells, compatible with tubulointerstitial nephritis (TIN). Immunohistochemical staining revealed FmoPV N protein-positive renal tubular cells and mononuclear cells in lymph nodes. A casecontrol study showed the presence of TIN in seven of 12 cats with FmoPV infection, but only two of 15 cats without FmoPV infection (P < 0.05), suggesting an association between FmoPV and TIN.
机译：我们描述了从家猫（Felis catus）副粘病毒，猫麻风病毒（FmoPV）的发现和分离。通过RT-PCR在457只流浪猫中的56只（12.3％）（53尿，四支直肠拭子和一份血样）中检测到FmoPV RNA。三个FmoPV菌株的完整基因组测序显示，基因组大小为16,050个碱基，在麻疹病毒中最大，这是因为400 nt的5'尾序列异常长。 FmoPV具有相同的基因含量（3-N-P / V / C-M-F-H-L-5），并与其他麻疹病毒在系统发育上成簇。 Western blot证实56只RT-PCR阳性猫的49血清（76.7％）中的抗FmoPV N蛋白IgG阳性，但401只RTPCR阴性猫中的78血清（19.4％）为阳性（P <0.0001）。从CRFK猫肾细胞中分离出FmoPV，通过细胞舍入，分离，裂解和合胞体形成引起细胞病变作用。 FmoPV还可以在灵长类Vero E6细胞的后续传代中复制。在对FmoPV N蛋白进行免疫染色时，感染的细胞系显示出细颗粒状和弥散性的胞质荧光。电子显微镜检查显示在副粘病毒中包膜病毒具有典型的“人字形”螺旋N。对两只FmoPV阳性猫的尸检组织进行组织学检查发现，肾中存在间质炎性浸润和肾小管变性/坏死，变性肾小管上皮细胞中的生长素表达降低，与肾小管间质性肾炎（TIN）相容。免疫组织化学染色显示淋巴结中FmoPV N蛋白阳性肾小管细胞和单核细胞。一项病例对照研究显示，在FmoPV感染的12只猫中有7只存在TIN，但15只FmoPV感染的猫中只有2只（P <0.05），表明FmoPV与TIN之间存在关联。

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    Patrick C. Y. Woo; Susanna K. P. Lau; Beatrice H. L. Wong; Rachel Y. Y. Fan; Annette Y. P. Wong; Anna J. X. Zhang; Ying Wu; Garnet K. Y. Choi; Kenneth S. M. Li; Janet Hui; Ming Wang; Bo-Jian Zheng; K. H. Chan; Kwok-Yung Yuen;

  • 作者单位

    State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Research Centre of Infection and Immunology, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Carol Yu Centre of Infection, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Research Centre of Infection and Immunology, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Carol Yu Centre of Infection, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    PathLab Medical Laboratories, Hong Kong;

    Guangzhou Center for Disease Control and Prevention, Guangzhou, China;

    State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Research Centre of Infection and Immunology, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Carol Yu Centre of Infection, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

    State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Department of Microbiology,University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Research Centre of Infection and Immunology, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China,Carol Yu Centre of Infection, University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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