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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Nascent RNA structure modulates the transcriptional dynamics of RNA polymerases
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Nascent RNA structure modulates the transcriptional dynamics of RNA polymerases

机译:新生的RNA结构调节RNA聚合酶的转录动力学

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摘要

Rna polymerase pausing represents an important mechanism of transcriptional regulation. In this study, we use a single-molecule transcription assay to investigate the effect of template base-pair composition on pausing by RNA polymerase II and the evolutiona-rily distinct mitochondrial polymerase Rpo41. For both enzymes, pauses are shorter and less frequent on GC-rich templates. Significantly, incubation with Rnase abolishes the template dependence of pausing. A kinetic model, wherein the secondary structure of the nascent RNA poses an energetic barrier to pausing by impeding backtracking along the template, quantitatively predicts the pause densities and durations observed. The energy barriers extracted from the data correlate well with RNA folding energies obtained from cotranscriptional folding simulations. These results reveal that RNA secondary structures provide a c/s-acting mechanism by which sequence modulates transcriptional elongation.
机译:Rna聚合酶暂停代表转录调控的重要机制。在这项研究中,我们使用单分子转录试验来研究模板碱基对组成对RNA聚合酶II和进化上独特的线粒体聚合酶Rpo41暂停的影响。对于这两种酶,在富含GC的模板上停顿都更短且更不频繁。显着地,与Rnase一起温育消除了暂停的模板依赖性。一种动力学模型,其中新生RNA的二级结构通过阻止沿模板的回溯而构成了暂停的能量屏障,定量预测了观察到的停顿密度和持续时间。从数据中提取的能垒与从共转录折叠模拟获得的RNA折叠能很好地相关。这些结果表明,RNA二级结构提供了一个c / s作用机制,该序列通过该机制调节转录伸长。

著录项

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  • 作者单位

    Jason L. Choy Laboratory of Single-Molecule Biophysics and Department of Physics, University of California, Berkeley, CA 94720;

    Jason L. Choy Laboratory of Single-Molecule Biophysics and Department of Physics, University of California, Berkeley, CA 94720;

    QB3 Institute, University of California, Berkeley, CA 94720;

    Jason L. Choy Laboratory of Single-Molecule Biophysics and Department of Physics, University of California, Berkeley, CA 94720 Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 Department of Chemistry, University of California, Berkeley, CA 94720 Howard Hughes Medical Institute, University of California, Berkeley, CA 94720;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    enzyme kinetics; pol II; transcriptional pausing; yeast;

    机译:酶动力学pol II;转录暂停;酵母;

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