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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Imaging electrical resonance in hair cells
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Imaging electrical resonance in hair cells

机译：成像毛细胞中的共振

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摘要

The mechanosensory hair cells of many auditory receptor organs are tuned by an electrical resonance that increases their responses to stimulation over a narrow band of frequencies. The small oscillations of membrane potential characteristic of this phenomenon have previously been detectable only through intracellular electrode measurements, which are laborious and preclude analysis at the level of an entire sensory organ. We used a voltage-sensitive dye to image hair-cell electrical resonance in an intact preparation of the bullfrog's sacculus, a receptor organ sensitive to low-frequency seismic and auditory stimuli. Imaging revealed distinct populations of hair cells whose resonant response varied with the frequency of transepithelial electrical stimulation. Most of the hair cells in the saccular epithelium in vitro were electrically tuned to stimulation at 25-50 Hz. The frequency dependence of the fluorescence signal was sensitive to pharmacological blockade of large-conductance Ca~(2+)-sensitive K~+ channels and to enzymatic digestion. At an elevated concentration of Ca~(2+), we observed transient fluorescence signals that probably represented action potentials. The stroboscopic imaging and analysis techniques described here present a general approach for studying subthreshold oscillations in electrically excitable cells.
机译：许多听觉受体器官的机械感官毛细胞是通过共振来调节的，该共振增加了它们在狭窄频带上对刺激的反应。以前仅通过细胞内电极测量就可以检测到这种现象的膜电位特征的小振荡，这是费力的并且排除了在整个感觉器官水平上的分析。在完整制备的牛蛙to囊（一种对低频地震和听觉刺激敏感的受体器官）中，我们使用了一种压敏染料来成像毛细胞的共振现象。成像显示出不同的毛细胞群，其共振响应随经上皮电刺激的频率而变化。电刺激体外囊状上皮中的大多数毛细胞在25-50 Hz刺激。荧光信号的频率依赖性对大电导Ca〜（2+）敏感的K〜+通道的药理阻滞和酶消化敏感。在高浓度的Ca〜（2+）下，我们观察到可能代表动作电位的瞬时荧光信号。这里描述的频闪成像和分析技术提供了一种研究电可激发细胞亚阈值振荡的通用方法。

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