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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Solute diffusion is hindered in the mitochondrial matrix
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Solute diffusion is hindered in the mitochondrial matrix

机译：线粒体基质中溶质扩散受到阻碍

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摘要

Intracellular chemical reactions generally constitute reaction-diffusion systems located inside nanostructured compartments like the cytosol, nucleus, endoplasmic reticulum, Golgi, and mitochondrion. Understanding the properties of such systems requires quantitative information about solute diffusion. Here we present a novel approach that allows determination of the solvent-dependent solute diffusion constant (D_(S0lvent)) inside cell compartments with an experimentally quantifiable nanostructure. In essence, our method consists of the.matching of synthetic fluorescence recovery after photobleaching (FRAP) curves, generated by a mathematical model with a realistic nanostructure, and experimental FRAP data. As a proof of principle, we assessed D_(S0lvent) of a monomeric fluorescent protein (AcGFP1) and its tandem fusion (AcGFP1~2) in the mitochondrial matrix of HEK293 cells. Our result's demonstrate that diffusion of both proteins is substantially slowed by barriers in the mitochondrial matrix (cristae), suggesting that cells can control the dynamics of biochemical reactions in this compartment by modifying its nanostructure.
机译：细胞内化学反应通常构成位于诸如细胞质，细胞核，内质网，高尔基体和线粒体等纳米结构隔室内的反应扩散系统。了解此类系统的特性需要有关溶质扩散的定量信息。在这里，我们提出了一种新颖的方法，该方法可以确定具有实验上可量化的纳米结构的细胞室内的溶剂依赖性溶质扩散常数（D_（Slvent））。从本质上讲，我们的方法包括对具有实际纳米结构的数学模型和实验FRAP数据进行光漂白（FRAP）曲线后合成荧光回收率的匹配。作为原理证明，我们评估了HEK293细胞线粒体基质中的单体荧光蛋白（AcGFP1）的D_（S0vent）及其串联融合（AcGFP1〜2）。我们的结果表明，线粒体基质（cristae）中的屏障会大大减慢这两种蛋白质的扩散，这表明细胞可以通过修饰其纳米结构来控制该区室中生化反应的动力学。

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  • 作者

    Cindy E. J. Dieteren; Stan C. A. M. Gielen; Leo G. J. Nijtmans; Jan A. M. Smeitink; Herman G. Swarts; Roland Brock; Peter H. G. M. Willems; Werner J. H. Koopman;

  • 作者单位

    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands,Department of Pediatrics, Nijmegen Centre for Mitochondrial Disorders, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

    Department of Biophysics, Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

    Department of Pediatrics, Nijmegen Centre for Mitochondrial Disorders, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

    Department of Pediatrics, Nijmegen Centre for Mitochondrial Disorders, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    molecular dynamics; quantitative random-walk model; systems biology;

    机译：分子动力学;定量随机游走模型;系统生物学;

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