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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Majority of CD4+ T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule
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Majority of CD4+ T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule

机译：HIV-1感染者外周血中的大多数CD4 + T细胞仅包含一个HIV DNA分子

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摘要

Neither the number of HIV-1 proviruses within individual infected cells in HIV-1-infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a tech nique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4+ T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, implying a limited potential for recombination in virus produced by these cells. The genetic similarity between HIV populations in CD4+ T cells and plasma implies ongoing exchange between these compartments both early and late after infection.
机译：HIV-1感染患者的单个感染细胞内HIV-1前病毒的数量及其在单个感染细胞内以及细胞与血浆病毒之间的遗传相关性都没有得到很好的定义。为了解决这些问题，我们开发了一种技术，可以对体内单个感染细胞中的HIV-1 DNA进行定量和遗传表征。对来自9位患者的外周血CD4 + T细胞的分析表明，大多数被感染的细胞仅包含一份HIV-1 DNA拷贝，这意味着这些细胞产生的病毒重组的潜力有限。 CD4 + T细胞和血浆中HIV人群之间的遗传相似性意味着感染后早期和晚期这些区室之间的持续交换。

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    Una Josefsson; Martin S. King; Barbro Makitalo; Johan Braennstroem; Wei Shao; Frank Maldarelli; Mary F. Kearney; Wei-Shau Hu; Jianbo Chen; Hans Gaines; John W. Mellors; Jan Albert; John M. Coffin; Sarah E. Palmer;

  • 作者单位

    Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden,Department of Microbiology, Tumor,and Cell Biology, Karolinska Instituted 171 82, Solna, Sweden,HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;

    Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60069;

    Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;

    Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;

    Advanced Biomedical Computing Center, SAIC Frederick, Inc.,Frederick, MD 21702;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;

    Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;

    Division of Infectious Diseases, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261;

    Department of Microbiology, Tumor,and Cell Biology, Karolinska Instituted 171 82, Solna, Sweden;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;

    Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden,Department of Microbiology, Tumor,and Cell Biology, Karolinska Instituted 171 82, Solna, Sweden;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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