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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Transcriptome transfer provides a model for understanding the phenotype of cardiomyocytes
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Transcriptome transfer provides a model for understanding the phenotype of cardiomyocytes

机译：转录组转移提供了一个了解心肌细胞表型的模型

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摘要

We show that the transfer of the adult ventricular myocyte (AVM) transcriptome into either a fibroblast or an astrocyte converts the host cell into a cardiomyocyte. Transcriptome-effected cardiomyocytes (tCardiomyocytes) display morphologies, immunocytochemi-cal properties, and expression profiles of postnatal cardiomyocytes. Cell morphology analysis shows that tCardiomyoctes are elongated and have a similar length-to-width ratio as AVMs. These global phenotypic changes occur in a time-dependent manner and confer electroexcitability to the tCardiomyocytes. tCardiomyocyte generation does not require continuous overexpression of specific transcription factors; for example, the expression level of transcription factor Mef2c is higher in tCardiomyocytes than in fibroblasts, but similar in tCardiomyocytes and AVMs. These data highlight the dominant role of the gene expression profile in developing and maintaining cellular phenotype. The transcriptome-induced phenotype remodeling-generated tCardiomyocyte has significant implications for understanding and modulating cardiac disease development.
机译：我们显示，成年成纤维细胞或星形胶质细胞的成年心室肌细胞（AVM）转录组的转移将宿主细胞转化为心肌细胞。转录组影响的心肌细胞（tCardiomyocytes）表现出形态，免疫细胞化学特性和产后心肌细胞的表达特征。细胞形态学分析显示，t心肌标记是延长的，长宽比与AVM相似。这些总体表型变化以时间依赖性方式发生，并赋予心肌细胞电兴奋性。心肌细胞的生成不需要特定转录因子的持续过表达；例如，转录因子Mef2c在心肌细胞中的表达水平高于成纤维细胞，但在心肌细胞和AVM中相似。这些数据突出了基因表达谱在发展和维持细胞表型中的主导作用。转录组诱导的表型重塑产生的心肌细胞对理解和调节心脏疾病的发展具有重要意义。

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  • 作者

    Tae Kyung Kim; Jai-Yoon Sul; Nataliya B. Peternko; Jae Hee Lee; Miler Lee; Vickas V. Patel; Junhyong Kim; James H. Eberwine;

  • 作者单位

    Department of Pharmacology, University of Pennsylvania Medical School, Philadelphia, PA 19104;

    Department of Pharmacology, University of Pennsylvania Medical School, Philadelphia, PA 19104;

    Penn Cardiovascular Institute and Division of Cardiovascular Medicine, University of Pennsylvania Medical School, Philadelphia, PA 19104;

    Department of Pharmacology, University of Pennsylvania Medical School, Philadelphia, PA 19104;

    Department of Biology , University of Pennsylvania Medical School, Philadelphia, PA 19104 Penn Genome Frontiers Institute, University of Pennsylvania Medical School, Philadelphia, PA 19104;

    Penn Cardiovascular Institute and Division of Cardiovascular Medicine, University of Pennsylvania Medical School, Philadelphia, PA 19104;

    Department of Biology , University of Pennsylvania Medical School, Philadelphia, PA 19104 Penn Genome Frontiers Institute, University of Pennsylvania Medical School, Philadelphia, PA 19104;

    Department of Pharmacology, University of Pennsylvania Medical School, Philadelphia, PA 19104 Department of Biology , University of Pennsylvania Medical School, Philadelphia, PA 19104 Penn Genome Frontiers Institute, University of Pennsylvania Medical School, Philadelphia, PA 19104;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    relative abundance; lipid-mediated; tiper; microarray; patch clamp;

    机译：相对丰度脂质介导的翻斗车微阵列膜片夹;

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