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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genome-wide landscape of polyadenylation in Arabidopsis provides evidence for extensive alternative polyadenylation
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Genome-wide landscape of polyadenylation in Arabidopsis provides evidence for extensive alternative polyadenylation

机译：全基因组在拟南芥中的聚腺苷酸化景观为广泛的替代性聚腺苷酸化提供了证据

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摘要

Alternative polyadenylation (APA) has been shown to play an important role in gene expression regulation in animals and plants. However, the extent of sense and antisense APA at the genome level is not known. We developed a deep-sequencing protocol that queries the junctions of 3'UTR and poly(A) tails and confidently maps the poly(A) tags to the annotated genome. The results of this mapping show that 70% of Arabidopsis genes use more than one poly(A) site, excluding microheterogeneity. Analysis of the poly(A) tags reveal extensive APA in introns and coding sequences, results of which can significantly alter transcript sequences and their encoding proteins. Although the interplay of intron splicing and polyadenylation potentially defines poly(A) site uses in introns, the polyadenylation signals leading to the use of CDS protein-coding region poly(A) sites are distinct from the rest of the genome. Interestingly, a large number of poly(A) sites correspond to putative antisense transcripts that overlap with the promoter of the associated sense transcript, a mode previously demonstrated to regulate sense gene expression. Our results suggest that APA plays a far greater role in gene expression in plants than previously expected.
机译：替代的聚腺苷酸化（APA）已显示在动植物的基因表达调控中起重要作用。但是，在基因组水平上有义和反义APA的程度尚不清楚。我们开发了一种深度测序协议，该协议可查询3'UTR和poly（A）尾部的连接点，并有把握地将poly（A）标签映射到带注释的基因组。该作图的结果表明，除微异质性外，70％的拟南芥基因使用一个以上的poly（A）位点。对poly（A）标签的分析揭示了内含子和编码序列中广泛的APA，其结果会显着改变转录物序列及其编码蛋白。尽管内含子剪接和聚腺苷酸化的相互作用可能定义了内含子中poly（A）位点的使用，但导致使用CDS蛋白编码区poly（A）位点的聚腺苷酸化信号却不同于基因组的其余部分。有趣的是，大量的poly（A）位点对应于推定的反义转录物，该转录物与相关的有义转录物的启动子重叠，该模式先前已证明可调节有义基因的表达。我们的结果表明，APA在植物基因表达中的作用比以前预期的要大得多。

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  • 作者

    Xiaohui Wu; Man Liu; Bruce Downie; Chun Liang; Guoli Ji; Qingshun Q. Li; Arthur G. Hunt;

  • 作者单位

    Department of Botany, Miami University, Oxford, OH 45056,Department of Automation, Xiamen University, Xiamen, Fujian 361005, People's Republic of China;

    Department of Botany, Miami University, Oxford, OH 45056;

    Department of Horticulture and Seed Biology Group, University of Kentucky, Lexington,KY 40546-0312;

    Department of Botany, Miami University, Oxford, OH 45056;

    Department of Automation, Xiamen University, Xiamen, Fujian 361005, People's Republic of China;

    Department of Botany, Miami University, Oxford, OH 45056,Department of Automation, Xiamen University, Xiamen, Fujian 361005, People's Republic of China;

    Department of Plant and Soil Sciences, University of Kentucky, Lexington,KY 40546-0312;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    alternative processing; antisense transcription; nonstop mrnas;

    机译：替代处理;反义转录;不间断;

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