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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Multilevel autoregulation of λ repressor protein CI by DNA looping in vitro
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Multilevel autoregulation of λ repressor protein CI by DNA looping in vitro

机译：体外通过DNA环化对λ阻遏蛋白CI进行多级自动调节

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摘要

The prophage state of bacteriophage X is extremely stable and is maintained by a highly regulated level of X repressor protein, Cl, which represses lytic functions. Cl regulates its own synthesis in a lysogen by activating and repressing its promoter, PRM. Cl participates in long-range interactions involving two regions of widely separated operator sites by generating a loop in the intervening DNA. We investigated the roles of each individual site under conditions that permitted DNA loop formation by using in vitro transcription assays for the first time on supercoiled DNA that mimics in vivo situation. We confirmed that DNA loops generated by oligo-merization of Cl bound to its operators influence the autoactiva-tion and autorepression of PRM regulation. We additionally report that different configurations of DNA loops are central to this regulation—one configuration further enhances autoactivation and another is essential for autorepression of PRM.
机译：噬菌体X的噬菌体状态极为稳定，并通过X阻遏蛋白Cl的高度调节水平来维持，该X阻遏蛋白C抑制裂解功能。 C1通过激活和抑制其启动子PRM来调节溶原中自身的合成。 C1通过在中间DNA中产生环而参与涉及广泛分开的操纵子位点的两个区域的远程相互作用。我们通过在模拟体内情况的超螺旋DNA上首次使用体外转录测定法，在允许DNA环形成的条件下研究了每个位点的作用。我们证实，通过绑定到其操纵子的Cl的寡聚产生的DNA环会影响PRM调控的自激活和自抑制。我们还报告说，不同的DNA环构型是该调控的核心-一种构型进一步增强了自动激活，而另一种构型对于PRM的自动阻遏至关重要。

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  • 作者

    Dale Lewis; Phuoc Le; Chiara Zurla; Laura Finzi; Sankar Adhya;

  • 作者单位

    Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4264;

    Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4264;

    Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

    Physics Department, 400 Dowman Drive,Emory University, Atlanta, GA 30322;

    Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4264;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    activation; cooperativity; repression;

    机译：激活;合作性抑制;

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