...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A quorum-sensing molecule acts as a morphogen controlling gas vesicle organelle biogenesis and adaptive flotation in an enterobacterium
【24h】

A quorum-sensing molecule acts as a morphogen controlling gas vesicle organelle biogenesis and adaptive flotation in an enterobacterium

机译：群体感应分子充当控制肠胃细菌中气泡细胞器生物发生和适应性漂浮的形态发生原

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gas vesicles are hollow intracellular proteinaceous organelles produced by aquatic Eubacteria and Archaea, including cyanobac-teria and halobacteria. Gas vesicles increase buoyancy and allow taxis toward air-liquid interfaces, enabling subsequent niche colonization. Here we report a unique example of gas vesicle-mediated flotation in an enterobacterium; Serratia sp. strain ATCC39006. This strain is a member of the Enterobacteriaceae previously studied for its production of prodigiosin and carbapenem antibiotics. Genes required for gas vesicle synthesis mapped to a 16.6-kb gene cluster encoding three distinct homologs of the main structural protein, GvpA. Heterologous expression of this locus in Escherkhia coli induced copious vesicle production and efficient cell buoyancy. Gas vesicle morphogenesis in Serratia enabled formation of a pellicle-like layer of highly vacuolated cells, which was dependent on oxygen limitation and the expression of ntrB/C and cheY-Wke regulatory genes within the gas-vesicle gene cluster. Gas vesicle biogenesis was strictly controlled by intercellular chemical signaling, through an W-acyl homoserine lactone, indicating that in this system the quorum-sensing molecule acts as a morphogen initiating organelle development. Flagella-based motility and gas vesicle morphogenesis were also oppositely regulated by the small RNA-binding protein, RsmA, suggesting environmental adaptation through physiological control of the choice between motility and flotation as alternative taxis modes. We propose that gas vesicle biogenesis in this strain represents a distinct mechanism of mobility, regulated by oxygen availability, nutritional status, the RsmA global regulatory system, and the quorum-sensing morphogen.
机译：气体囊泡是由水生真细菌和古细菌产生的中空细胞内蛋白质细胞器，包括蓝细菌和嗜盐菌。气体小泡增加了浮力，并使滑行朝向气液界面，从而使随后的利基菌落得以定殖。在这里，我们报告了在肠杆菌中气体囊泡介导的浮选的独特例子。沙雷氏菌菌株ATCC39006。该菌株是肠杆菌科的成员，先前曾对其产生prodigiosin和碳青霉烯类抗生素进行过研究。气体囊泡合成所需的基因定位到编码主要结构蛋白GvpA的三个不同同源物的16.6kb基因簇。该位点在大肠杆菌中的异源表达诱导大量囊泡的产生和有效的细胞浮力。沙雷氏菌中的气体囊泡形态发生使得能够形成高度空泡的细胞的膜状层，这取决于氧限制以及气体囊泡基因簇内ntrB / C和cheY-Wke调节基因的表达。气体囊泡的生物发生受到细胞间化学信号的严格控制，通过W-酰基高丝氨酸内酯，表明在该系统中，群体感应分子充当启动细胞器发育的形态发生子。鞭毛为基础的运动性和气体囊泡的形态发生也受到小RNA结合蛋白RsmA的相反调节，这表明通过生理控制运动性和浮选之间的选择作为滑行的替代方式，可以适应环境。我们建议该菌株中的气体囊泡生物发生代表了一种独特的流动性机制，受氧气供应，营养状况，RsmA全球调节系统和群体感应形态发生素的调控。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号