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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genome-wide antisense transcription drives mRNA processing in bacteria
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Genome-wide antisense transcription drives mRNA processing in bacteria

机译：全基因组反义转录驱动细菌中的mRNA加工

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摘要

RNA deep sequencing technologies are revealing unexpected levels of complexity in bacterial transcriptomes with the discovery of abundant noncoding RNAs, antisense RNAs, long 5' and 3' untranslated regions, and alternative operon structures. Here, by applying deep RNA sequencing to both the long and short RNA fractions (<50 nucleotides) obtained from the major human pathogen Staphylo-coccus aureus, we have detected a collection of short RNAs that is generated genome-wide through the digestion of overlapping sense/antisense transcripts by RNase Ⅲ endoribonuclease. At least 75% of sense RNAs from annotated genes are subject to this mechanism of antisense processing. Removal of RNase Ⅲ activity reduces the amount of short RNAs and is accompanied by the accumulation of discrete antisense transcripts. These results suggest the production of pervasive but hidden antisense transcription used to process sense transcripts by means of creating double-stranded substrates. This process of RNase Ⅲ-mediated digestion of overlapping transcripts can be observed in several evolutionarily diverse Gram-positive bacteria and is capable of providing a unique genome-wide posttranscriptional mechanism to adjust mRNA levels.
机译：RNA深度测序技术揭示了细菌转录组中意想不到的复杂性水平，发现了丰富的非编码RNA，反义RNA，长5'和3'非翻译区以及其他操纵子结构。在这里，通过对主要人类病原体金黄色葡萄球菌获得的长和短RNA片段（<50个核苷酸）进行深度RNA测序，我们检测到了短RNA的集合，这些短RNA是通过消化重叠而在全基因组范围内产生的核糖核酸酶Ⅲ核糖核酸酶的有义/反义转录本。来自注释基因的有义RNA至少有75％受到这种反义加工机制的影响。 RNaseⅢ活性的去除减少了短RNA的数量，并伴随着离散的反义转录物的积累。这些结果表明产生了普遍的但隐藏的反义转录，用于通过产生双链底物来处理有义转录物。 RNaseⅢ介导的重叠转录物的消化过程可在几种进化多样的革兰氏阳性细菌中观察到，并能够提供独特的全基因组转录后机制来调节mRNA水平。

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    Inigo Lasa; Alejandro Toledo-Arana; Alexander Dobin; Maite Villanueva; Igor Ruiz de los Mozos; Marta Vergara-lrigaray; Victor Segura; Delphine Fagegaltier; Jose R. Penades; Jaione Valle; Cristina Solano; Thomas R. Gingeras;

  • 作者单位

    Laboratory of Microbial Biofilms, Institute de Agrobiotecnologia, Consejo Superior de Investigaciones Cientfficas-Universidad Publica de Navarra-Gobierno de Navarra, 31006 Pamplona, Spain contributed equally to this work;

    Laboratory of Microbial Biofilms, Institute de Agrobiotecnologia, Consejo Superior de Investigaciones Cientfficas-Universidad Publica de Navarra-Gobierno de Navarra, 31006 Pamplona, Spain contributed equally to this work;

    Laboratory of Functional Genomics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724;

    Laboratory of Microbial Biofilms, Institute de Agrobiotecnologia, Consejo Superior de Investigaciones Cientfficas-Universidad Publica de Navarra-Gobierno de Navarra, 31006 Pamplona, Spain contributed equally to this work;

    Laboratory of Microbial Biofilms, Institute de Agrobiotecnologia, Consejo Superior de Investigaciones Cientfficas-Universidad Publica de Navarra-Gobierno de Navarra, 31006 Pamplona, Spain contributed equally to this work;

    Laboratory of Microbial Biofilms, Institute de Agrobiotecnologia, Consejo Superior de Investigaciones Cientfficas-Universidad Publica de Navarra-Gobierno de Navarra, 31006 Pamplona, Spain contributed equally to this work;

    Genomics,Proteomics and Bioinformatics Unit, Centra de Investigation Medica Aplicada, Universidad de Navarra, 31008 Pamplona, Spain;

    Laboratory of Functional Genomics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724;

    lnstituto en Ganaderia de Montana-Consejo Superior de Investigaciones Cientificas, 24346 Leon, Spain;

    Laboratory of Microbial Biofilms, Institute de Agrobiotecnologia, Consejo Superior de Investigaciones Cientfficas-Universidad Publica de Navarra-Gobierno de Navarra, 31006 Pamplona, Spain contributed equally to this work;

    Laboratory of Microbial Biofilms, Institute de Agrobiotecnologia, Consejo Superior de Investigaciones Cientfficas-Universidad Publica de Navarra-Gobierno de Navarra, 31006 Pamplona, Spain contributed equally to this work;

    Laboratory of Functional Genomics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    antisense RNA; overlapping transcription; RNA processing; posttranscriptional regulation; microRNA;

    机译：反义RNA重叠转录;RNA加工;转录后调控;微小RNA;

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