Central role of the RNA polymerase trigger loop in intrinsic RNA hydrolysis

Yulia Yuzenkova; rnNikolay Zenkin

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Central role of the RNA polymerase trigger loop in intrinsic RNA hydrolysis

机译：RNA聚合酶触发环在固有RNA水解中的核心作用

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The active center of RNA polymerase can hydrolyze phospho-diester bonds in nascent RNA, a reaction thought to be important for proofreading of transcription. The reaction proceeds via a general two Mg~(2+) mechanism and is assisted by the 3' end nucleotide of the transcript. Here, by using Thermus aquaticus RNA polymerase, we show that the reaction also requires the flexible domain of the active center, the trigger loop (TL). We show that the invariant histidine (β' His1242) of the TL is essential for hydrolysis/proofreading and participates in the reaction in two distinct ways: by positioning the 3' end nucleotide of the transcript that assists catalysis and/or by directly participating in the reaction as a general base. We also show that participation of the p' His1242 of the TL in phosphodiester bond hydrolysis does not depend on the extent of elongation complex backtracking. We obtained similar results with Escherichia coli RNA polymerase, indicating that the function of the TL in phosphodiester bond hydrolysis is conserved among bacteria.

机译：RNA聚合酶的活性中心可以水解新生RNA中的磷酸二酯键，这种反应被认为对转录的校对很重要。该反应通过一般的两个Mg〜（2+）机制进行，并由转录本的3'末端核苷酸协助。在这里，通过使用水生栖热菌RNA聚合酶，我们表明该反应还需要活性中心的柔性域，即触发环（TL）。我们显示，TL的不变组氨酸（β'His1242）对于水解/校对是必不可少的，并以两种不同的方式参与反应：通过定位有助于催化的转录本的3'末端核苷酸和/或直接参与反应是一般的基础。我们还显示，在磷酸二酯键水解中TL的p'His1242的参与不取决于延伸复合物回溯的程度。我们用大肠杆菌RNA聚合酶获得了相似的结果，表明TL在磷酸二酯键水解中的功能在细菌之间是保守的。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2010年第24期|P.10878-10883|共6页
作者
Yulia Yuzenkova; rnNikolay Zenkin;
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作者单位

Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, NE2 4AX, United Kingdom;

rnCentre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, NE2 4AX, United Kingdom;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
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6. Central role of the RNA polymerase trigger loop in intrinsic RNA hydrolysis [O] . Yulia Yuzenkova, Nikolay Zenkin 2010

机译：RNA聚合酶触发环在固有RNA水解中的核心作用
7. Central role of the RNA polymerase trigger loop in intrinsic RNA hydrolysis [O] . Yuzenkova, Yulia, Zenkin, Nikolay 2010

机译：RNA聚合酶触发环在固有RNA水解中的核心作用

Central role of the RNA polymerase trigger loop in intrinsic RNA hydrolysis

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