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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Source identification in two criminal cases using phylogenetic analysis of HIV-1 DNA sequences
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Source identification in two criminal cases using phylogenetic analysis of HIV-1 DNA sequences

机译:使用HIV-1 DNA序列的系统发育分析鉴定两个刑事案件的来源

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摘要

Phylogenetic analysis has been widely used to test the a priori hypothesis of epidemiological clustering in suspected transmission chains of HIV-1. Among studies showing strong support for relatedness between HIV samples obtained from infected individuals, evidence for the direction of transmission between epidemi-ologically related pairs has been lacking. During transmission of HIV, a genetic bottleneck occurs, resulting in the paraphyly of source viruses with respect to those of the recipient. This paraphyly establishes the direction of transmission, from which the source can then be inferred. Here, we present methods and results from two criminal cases, State of Washington v Anthony Eugene Whitfield, case number 04-1-0617-5 (Superior Court of the State of Washington, Thurston County, 2004) and State of Texas v Philippe Padieu, case numbers 219-82276-07, 219-82277-07, 219-82278-07, 219-82279-07, 219-82280-07, and 219-82705-07 (219th Judicial District Court, Collin County, TX, 2009), which provided evidence that direction can be established from blinded case samples. The observed paraphyly from each case study led to the identification of an inferred source (i.e., index case), whose identity was revealed at trial to be that of the defendant.
机译:系统发育分析已被广泛用于检验HIV-1疑似传播链中流行病学聚类的先验假设。在研究中强烈支持从感染个体获得的艾滋病毒样本之间的相关性的研究中,缺乏流行病学相关对之间传播方向的证据。在HIV传播过程中,发生了遗传瓶颈,导致源病毒相对于受者的寄生虫。这从理论上确定了传播方向,然后可以从中推断出来源。在这里,我们提供两种刑事案件的方法和结果,分别是华盛顿州诉安东尼·尤金·惠特菲尔德,案件编号04-1-0617-5(华盛顿州高级法院,瑟斯顿县,2004年)和德克萨斯州诉菲利普·帕迪亚,案件编号219-82276-07、219-82277-07、219-82278-07、219-82279-07、219-82280-07和219-82705-07(德克萨斯州科林县219级司法法院, 2009年),这提供了可以从盲目案例中确定方向的证据。从每个案例研究中观察到的副学特征导致鉴定出一个推断出的来源(即索引案例),该来源在审判中被揭示为被告。

著录项

  • 来源
  • 作者单位

    Human Genome Sequencing Center,Baylor College of Medicine,Houston, TX 77030,Department of Molecular and Human Genetics,Baylor College of Medicine,Houston, TX 77030;

    Section of Integrative Biology and Center for Computational Biology and Bioinformatics, University of Texas, Austin, TX 78712;

    Human Genome Sequencing Center,Baylor College of Medicine,Houston, TX 77030,Department of Molecular and Human Genetics,Baylor College of Medicine,Houston, TX 77030;

    Section of Integrative Biology and Center for Computational Biology and Bioinformatics, University of Texas, Austin, TX 78712;

    Section of Integrative Biology and Center for Computational Biology and Bioinformatics, University of Texas, Austin, TX 78712;

    Human Genome Sequencing Center,Baylor College of Medicine,Houston, TX 77030,Department of Molecular and Human Genetics,Baylor College of Medicine,Houston, TX 77030,Cell and Molecular Biology Program, Baylor College of Medicine,Houston, TX 77030;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    HIV transmission; phylogeny; forensics; evolution; molecular epidemiology;

    机译:艾滋病毒传播;系统发育;法医;演变;分子流行病学;

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