...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration
【24h】

Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration

机译：通过极化的内吞循环空间限制受体酪氨酸激酶活性控制边界细胞迁移

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Border cell migration is a stereotyped migration occurring during the development of the Drosophila egg chamber. During this process, a cluster composed of six to eight follicle cells migrates between nurse cells toward the oocyte. Receptor tyrosine kinases (RTKs) are enriched at the leading edge of the follicle cells and establish the directionality of their migration. Endocytosis has been shown to play a role in the maintenance of this polarization; however, the mechanisms involved are largely unknown. In this study, we show that border cell migration requires the function of the small GTPases Rab5 and Rab11 that regulate trafficking through the early and the recycling endosome, respectively. Expression of a dominant negative form of rab 11 induces a loss of the polarization of RTK activity, which correlates with a severe migration phenotype. In addition, we demonstrate that the exocyst component Sec15 is distributed in structures that are polarized during the migration process in a Rab11 -dependent manner and that the down-regulation of different subunits of the exocyst also affects migration. Together, our data demonstrate a fundamental role for a plasma membrane-endosome trafficking cycle in the maintenance of active RTK at the leading edge of border cells during their migration.
机译：边界细胞迁移是在果蝇卵室发育期间发生的定型迁移。在此过程中，由六到八个卵泡细胞组成的簇在护士细胞之间向卵母细胞迁移。受体酪氨酸激酶（RTKs）在卵泡细胞的前缘富集，并确定其迁移的方向性。已经证明，胞吞作用在维持这种极化中起着作用。但是，所涉及的机制很大程度上未知。在这项研究中，我们表明边界细胞迁移需要小的GTPases Rab5和Rab11的功能，它们分别调节通过早期和回收内体的运输。 rab11的显性负型表达引起RTK活性极化的丧失，这与严重的迁移表型相关。此外，我们证明了囊外成分Sec15以Rab11依赖性方式在迁移过程中极化的结构中分布，并且囊外不同亚基的下调也影响迁移。总之，我们的数据证明了质膜-内体运输周期在迁移过程中维持边界细胞前沿的活性RTK的基本作用。

著录项

  • 来源
  • 作者

    Gloria Assaker; Damien Ramel; Stefanie K. Wculek; Marcos Gonzalez-Gaitan; Gregory Emery;

  • 作者单位

    Institute for Research in Immunology and Cancer and Department of Pathology and Cell Biology, Faculty of Medicine, Universite de Montreal, Montreal,Quebec, Canada H3C 3J7;

    Institute for Research in Immunology and Cancer and Department of Pathology and Cell Biology, Faculty of Medicine, Universite de Montreal, Montreal,Quebec, Canada H3C 3J7;

    Institute for Research in Immunology and Cancer and Department of Pathology and Cell Biology, Faculty of Medicine, Universite de Montreal, Montreal,Quebec, Canada H3C 3J7;

    Department of Biochemistry, Universite de Geneve, 1211 Geneva 4, Switzerland;

    Institute for Research in Immunology and Cancer and Department of Pathology and Cell Biology, Faculty of Medicine, Universite de Montreal, Montreal,Quebec, Canada H3C 3J7;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    vesicular trafficking;

    机译：水泡运输;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号