...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Multifunctionality And Mechanism Of Ligand Binding In A Mosquito Antiinflammatory Protein
【24h】

Multifunctionality And Mechanism Of Ligand Binding In A Mosquito Antiinflammatory Protein

机译：蚊子抗炎蛋白中配体结合的多功能性和机理

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mosquito D7 salivary proteins are encoded by a multigene family related to the arthropod odorant-binding protein (OBP) super-family. Forms having either one or two OBP domains are found in mosquito saliva. Four single-domain and one two-domain D7 proteins from Anopheles gambiae and Aedes aegypti (AeD7), respectively, were shown to bind biogenic amines with high affinity and with a stoi-chiometry of one ligand per protein molecule. Sequence comparisons indicated that only the C-terminal domain of AeD7 is homologous to the single-domain proteins from A gambiae, suggesting that the N-terminal domain may bind a different class of ligands. Here, we describe the 3D structure of AeD7 and examine the ligand-binding characteristics of the N- and C-terminal domains. Isothermal titration calorimetry and ligand complex crystal structures show that the N-terminal domain binds cysteinyl leukotrienes (cysLTs) with high affinities (50-60 nM) whereas the C-terminal domain binds biogenic amines. The lipid chain of the cysLT binds in a hydrophobic pocket of the N-terminal domain, whereas binding of norepinephrine leads to an ordering of the C-terminal portion of the C-terminal domain into an α-helix that, along with rotations of Arg-176 and Glu-268 side chains, acts to bury the bound ligand.
机译：蚊子D7唾液蛋白由与节肢动物气味结合蛋白（OBP）超家族有关的多基因家族编码。在蚊子唾液中发现具有一个或两个OBP结构域的形式。分别来自冈比亚按蚊和埃及伊蚊（AeD7）的四个单域和一个两个域D7蛋白（AeD7）以高亲和力和每个蛋白质分子一个配体的立体化学结合生物胺。序列比较表明，只有AeD7的C末端结构域与来自冈比亚的单结构域蛋白同源，这表明N末端结构域可以结合不同种类的配体。在这里，我们描述了AeD7的3D结构，并检查了N和C末端域的配体结合特征。等温滴定热法和配体络合物晶体结构表明，N末端结构域以高亲和力（50-60 nM）结合半胱氨酰白三烯（cysLTs），而C末端结构域则结合生物胺。 cysLT的脂质链结合在N末端结构域的疏水口袋中，而去甲肾上腺素的结合导致C末端结构域的C末端部分排列成α螺旋，随着Arg的旋转-176和Glu-268侧链可掩埋结合的配体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号