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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis
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A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis

机译：骨干连体分析精确描绘了大肠杆菌O157的出现和受限制的辐射

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摘要

Single nucleotide polymorphisms (SNPs) in stable genome regions provide durable measurements of species evolution. We systematically identified each SNP in concatenations of all backbone ORFs in 7 newly or previously sequenced evolutionarily instructive pathogenic Escherichia coli O157:H7, O157:H~-, and O55:H7. The 1,113 synonymous SNPs demonstrate emergence of the largest cluster of this pathogen only in the last millennium. Unexpectedly, shared SNPs within circumscribed clusters of organisms suggest severely restricted survival and limited effective population sizes of pathogenic O157:H7, tenuous survival of these organisms in nature, source-sink evolutionary dynamics, or, possibly, a limited number of mutations that confer selective advantage. A single large segment spanning the rfb-gnd gene cluster is the only backbone region convincingly acquired by recombination as O157 emerged from 055. This concatenomic analysis also supports using SNPs to differentiate closely related pathogens for infection control and forensic purposes. However, constrained radiations raise the possibility of making false associations between isolates.
机译：稳定基因组区域中的单核苷酸多态性（SNP）提供了物种进化的持久测量。我们系统地鉴定了7个新近或先前测序的具有进化指导意义的致病性大肠杆菌O157：H7，O157：H〜-和O55：H7中所有主链ORF的串联中的每个SNP。 1,113个同义SNP仅在最近的千年中才显示出这种病原体的最大簇。出乎意料的是，受限制的生物集群中共享的SNPs提示病原体O157：H7的生存受到严格限制，有效种群数量受到限制，这些生物在自然界中的生存能力很弱，源库进化动态，或者可能是有限数量的赋予选择性的突变优点。当从055出现O157时，跨越rfb-gnd基因簇的单个大片段是令人信服的唯一骨架区域。重组分析也支持使用SNP区分密切相关的病原体，以进行感染控制和法医鉴定。但是，受约束的辐射增加了在分离株之间产生假关联的可能性。

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    Shana R. Leopold; Vincent Magrini; Nicholas J. Holt; Nurmohammad Shaikh; Elaine R. Mardis; Joseph Cagno; Yoshitoshi Ogura; Atsushi lguchi; Alexander Mellmanns; Helge Karchs; Thomas E. Besser; Stanley A. Sawyer; Thomas S. Whittam; Phillip I. Tarr;

  • 作者单位

    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110 Molecular Microbiology and Microbial Pathogenesis Program, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110;

    Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park Avenue, Saint Louis, MO 63108;

    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110;

    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110;

    Genome Sequencing Center, Washington University School of Medicine, 4444 Forest Park Avenue, Saint Louis, MO 63108;

    Children's Hospital and Regional Medical Center, P.O. Box 5371, Seattle, WA 98105 Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan;

    Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan;

    Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan;

    Institute of Hygiene, University of Munster, Robert-Koch-Strasse 41, 48149 Munster, Germany Interdisciplinary Center for Clinical Research, Munster, Domagkstrasse 3, 48149 Munster, Germany;

    Institute of Hygiene, University of Munster, Robert-Koch-Strasse 41, 48149 Munster, Germany Interdisciplinary Center for Clinical Research, Munster, Domagkstrasse 3, 48149 Munster, Germany;

    Department of Veterinary Microbiology and Pathology, Washington State University, 402 Bustad Hall, Pullman, WA 99164;

    Department of Mathematics, Washington University, St. Louis, MO 63130;

    Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824;

    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    E. coli; evolution; SNPs;

    机译：大肠杆菌;演化;单核苷酸多态性;

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