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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading
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Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading

机译：丝氨酸整合酶介导切除的两步位点选择：DNA定向整合酶构象和中央二核苷酸校对

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摘要

Bacteriophage-encoded serine-integrases are members of the large family of serine-recombinases and catalyze site-specific integrative recombination between a phage attP site and a bacterial attB site to form an integrated prophage. Prophage excision involves a second site-specific recombination event, in which the sites generated by integration, attL and attR, are used as substrates to regenerate attP and attB. Excision is catalyzed by integrase but also requires a phage-encoded recombination directionality factor (RDF). The Bxb1 recombination sites, attP and attB, are small (<50 bp), different in sequence, and quasi-symmetrical, and they give rise to attL- and attR-recombinant products that are asymmetric but similar to each other, each being composed of B- and P-type half-sites. We show here that the determination of correct excision products is a two-step process, with a presynaptic RDF-dependent step that aligns attL and attR in the correct orientation and a postsynaptic step in which the nonpalindromic central dinucleotide confers identity to attL and attR and prevents each from recombining with itself.
机译：噬菌体编码的丝氨酸整合酶是丝氨酸重组酶大家族的成员，并催化噬菌体attP位点和细菌attB位点之间的位点特异性整合重组，以形成整合的噬菌体。噬菌体切除涉及第二个位点特异性重组事件，其中整合，attL和attR产生的位点被用作底物来再生attP和attB。整合酶催化切除，但还需要噬菌体编码的重组方向因子（RDF）。 Bxb1重组位点attP和attB小（<50 bp），序列不同，并且是准对称的，它们产生不对称但彼此相似但彼此组成的atL和atR重组产物B型和P型半位。我们在这里显示，确定正确的切除产物是一个两步过程，其中突触前RDF依赖性步骤将atL和atR对准正确的方向，而突触后步骤则使非回文中央二核苷酸赋予attL和attR相同性，并且防止每个人与自己重组。

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