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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789
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Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789

机译：酿酒酵母YJM789基因组测序与比较分析

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摘要

We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensive phenotypic differences to the laboratory reference strain, including growth at high temperature and deadly virulence in mouse models. Here we show that the ≈12-Mb genome of YJM789 contains ≈60,000 SNPs and ≈6,000 indels with respect to the reference S288c genome, leading to protein polymorphisms with a few known cases of phenotypic changes. Several ORFs are found to be unique to YJM789, some of which might have been acquired through horizontal transfer. Localized regions of high polymorphism density are scattered over the genome, in some cases spanning multiple ORFs and in others concentrated within single genes. The sequence of YJM789 contains clues to pathogenicity and spurs the development of more powerful approaches to dissecting the genetic basis of complex hereditary traits.
机译：我们对酿酒酵母菌株YJM789的基因组进行了测序，该菌株来源于酵母菌，该酵母菌是从患有艾滋病的肺炎患者的肺中分离出来的。由于该菌株与实验室参考菌株存在广泛的表型差异，包括高温下的生长和小鼠模型中的致命毒力，因此该菌株用于研究真菌感染和定量遗传学。在这里，我们显示YJM789的≈12-Mb基因组相对于参考S288c基因组包含≈60,000个SNP和≈6,000个indel，从而导致蛋白质多态性以及一些已知的表型改变。发现有几个ORF对于YJM789是唯一的，其中一些可能是通过水平传输获得的。高多态性密度的局部区域散布在基因组上，在某些情况下跨越多个ORF，而在另一些情况下则集中在单个基因内。 YJM789的序列包含了致病性的线索，并促使人们开发出更强大的方法来解析复杂的遗传性状的遗传基础。

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