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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Protection of telomeres by a conserved Stn1-Ten1 complex
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Protection of telomeres by a conserved Stn1-Ten1 complex

机译：保守的Stn1-Ten1复合物保护端粒

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摘要

Telomeres are specialized chromatin structures that protect chromosome ends. Critical among telomere proteins are those that bind the telomeric single-strand DNA (ssDNA) overhangs. These proteins are thought to differ among eukaryotes. Three interacting proteins (Cdc13, Stn1, and Ten1) associate with the telomeric overhang in budding yeast, a single protein known as Pot1 (protection of telomeres-1) performs this function in fission yeast, and a two-subunit complex consisting of POT1 and TPP1 associates with telomeric ssDNA in humans. Cdc13 and Pot1 have related oligonucleotide/oligosaccharide-binding fold (OB-fold) domains that bind the telomeric ssDNA overhang. Here we show that Schizosaccharomyces pombe has Stn1- and Ten1-like proteins that are essential for chromosome end protection. Stn1 orthologs exist in all species that have Pot1, whereas Ten1-like proteins can be found in all fungi. Fission yeast Stn1 and Ten1 localize at telomeres in a manner that correlates with the length of the ssDNA overhang, suggesting that they specifically associate with the telomeric ssDNA. Unlike in budding yeast, in which Cdc13, Stn1, and Ten1 all interact, fission yeast Stn1 and Ten1 associate with each other, but not with Pot1. Our findings suggest that two separate protein complexes are required for chromosome end protection in fission yeast. Structural profiling studies detect OB-fold domains in Stn1 and Ten1 orthologs, indicating that protection of telomeres by multiple proteins with OB-fold domains is conserved in eukaryotic evolution.
机译：端粒是专门的染色质结构，可保护染色体末端。端粒蛋白中的关键是那些结合端粒单链DNA（ssDNA）突出端的蛋白。这些蛋白质被认为在真核生物之间是不同的。三种相互作用的蛋白质（Cdc13，Stn1和Ten1）与发芽酵母中的端粒突出端相关，一种称为Pot1（端粒-1的保护）的蛋白质在裂变酵母中执行此功能，并且由POT1和TPP1与人类端粒ssDNA相关。 Cdc13和Pot1具有相关的寡核苷酸/寡糖结合折叠（OB-fold）域，它们结合端粒ssDNA突出端。在这里，我们显示粟酒裂殖酵母具有Stn1和Ten1样蛋白，它们对染色体末端保护至关重要。 Stn1直系同源物存在于所有具有Pot1的物种中，而Ten1样蛋白可以在所有真菌中找到。裂变酵母Stn1和Ten1以与ssDNA突出端的长度相关的方式定位于端粒，表明它们与端粒ssDNA特异性结合。与在芽孢酵母中Cdc13，Stn1和Ten1都相互作用的不同，裂变酵母Stn1和Ten1彼此关联，但不与Pot1关联。我们的发现表明，裂变酵母中的染色体末端保护需要两个单独的蛋白质复合物。结构分析研究检测到Stn1和Ten1直系同源物中的OB折叠结构域，表明真核生物进化过程中保守了具有OB折叠结构域的多种蛋白质对端粒的保护。

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