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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The oxysterol binding protein Kes1p regulates Golgi apparatus phosphatidylinositol-4-phosphate function
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The oxysterol binding protein Kes1p regulates Golgi apparatus phosphatidylinositol-4-phosphate function

机译：氧固醇结合蛋白Kes1p调节高尔基体磷脂酰肌醇-4-磷酸酯的功能

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摘要

The Saccharomyces cerevisiae phosphatidylcholine/phosphatidyl-inositol transfer protein Sec14p is required for Golgi apparatus-derived vesicular transport through coordinate regulation of phos-pholipid metabolism. Sec14p is normally essential. The essential requirement for SEC14 can be bypassed by inactivation of (ⅰ) the CDP-choline pathway for phosphatidylcholine synthesis or (ⅱ) KES1, which encodes an oxysterol binding protein. A unique screen was used to determine genome-wide genetic interactions for the essential gene SEC14 and to assess whether the two modes of "sec14 bypass" were similar or distinct. The results indicate that inactivation of the CDP-choline pathway allows cells with inactivated SEC14 to live through a mechanism distinct from that of inactivation of KES1. We go on to demonstrate an important biological function of Kesip. Kesip regulates Golgi apparatus-derived vesicular transport by inhibiting the function of Pik1p-generated Golgi apparatus phosphatidylinositol-4-phosphate (Pl-4P). Kes1p affects both the availability and level of Golgi apparatus PI-4P. A set of potential PI-4P-responsive proteins that include the Rab GTPase Ypt31p and its GTP exchange factor are described.
机译：啤酒酵母的磷脂酰胆碱/磷脂酰肌醇转移蛋白Sec14p是高尔基体来源的囊泡转运蛋白，通过协调调节磷脂代谢而需要。 Sec14p通常是必不可少的。 SEC14的基本要求可以通过灭活（the）磷脂酰胆碱合成的CDP-胆碱途径或（ⅱ）编码氧固醇结合蛋白的KES1来绕过。独特的筛选用于确定必需基因SEC14的全基因组遗传相互作用，并评估“ sec14旁路”的两种模式是相似还是不同。结果表明，CDP-胆碱途径的失活使SEC14失活的细胞能够通过不同于KES1失活的机制存活。我们继续证明Kesip的重要生物学功能。 Kesip通过抑制Pik1p生成的高尔基体磷脂酰肌醇-4-磷酸酯（Pl-4P）的功能来调节高尔基体来源的囊泡运输。 Kes1p影响高尔基体PI-4P的可用性和水平。描述了一组潜在的PI-4P反应蛋白，包括Rab GTPase Ypt31p及其GTP交换因子。

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