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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories
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Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories

机译：血凝素在40 nm处在活细胞膜中的动态聚集分布可区分筏理论

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摘要

Organization in biological membranes spans many orders of magnitude in length scale, but limited resolution in far-field light microscopy has impeded distinction between numerous biomem-brane models. One canonical example of a heterogeneously distributed membrane protein is hemagglutinin (HA) from influenza virus, which is associated with controversial cholesterol-rich lipid rafts. Using fluorescence photoactivation localization microscopy, we are able to image distributions of tens of thousands of HA molecules with subdiffraction resolution (≈ 40 nm) in live and fixed fibroblasts. HA molecules form irregular clusters on length scales from ≈ 40 nm up to many micrometers, consistent with results from electron microscopy. In live cells, the dynamics of HA molecules within clusters is observed and quantified to determine an effective diffusion coefficient. The results are interpreted in terms of several established models of biological membranes.
机译：生物膜的组织结构在长度尺度上跨越多个数量级，但是远场光学显微镜的有限分辨率阻碍了众多生物膜模型之间的区别。膜蛋白异质分布的一个典型例子是来自流感病毒的血凝素（HA），它与有争议的富含胆固醇的脂筏有关。使用荧光光活化定位显微镜，我们能够对成千上万个具有亚衍射分辨率（≈40 nm）的HA分子在活的和固定的成纤维细胞中的分布进行成像。 HA分子在约40 nm到许多微米的长度范围内形成不规则簇，这与电子显微镜的结果一致。在活细胞中，观察并量化了簇中HA分子的动力学，以确定有效的扩散系数。根据几种已建立的生物膜模型来解释结果。

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