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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mating-responsive genes in reproductive tissues of female Drosophila melanogaster
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Mating-responsive genes in reproductive tissues of female Drosophila melanogaster

机译：女性果蝇生殖组织中的交配反应基因

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Male-derived accessory gland proteins that are transferred to females during mating have profound effects on female reproductive physiology including increased ovulation, mating inhibition, and effects on sperm utilization and storage. The extreme rates of evolution seen in accessory gland proteins may be driven by sperm competition and sexual conflict, processes that may ultimately drive complex interactions between female- and male-derived molecules and sperm. However, little is known of how gene expression in female reproductive tissues changes in response to the presence of male molecules and sperm. To characterize this response, we conducted parallel genomic and proteomic analyses of gene expression in the reproductive tract of 3-day-old unmated and mated female Drosophila melanogaster. Using DNA microarrays, we identified 539 transcripts that are differentially expressed in unmated vs. mated females and revealed a striking peak in differential expression at 6 h postmating and a marked shift from primarily down-regulated to primarily up-regulated transcripts within 3 h after mating. Combining two-dimensional gel electrophoresis and liquid chromatography mass spectrometry analyses, we identified 84 differentially expressed proteins at 3 h postmating, including proteins that appeared to undergo posttranslational modification. Together, our observations define transcriptional and translational response to mating within the female reproductive tract and suggest a bimodal model of postmating gene expression initially correlated with mating and the final stages of female reproductive tract maturation and later with the declining presence of male reproductive molecules and with sperm maintenance and utilization.
机译：在交配过程中转移至雌性的雄性来源的辅助腺蛋白对雌性生殖生理具有深远的影响，包括排卵增加，交配抑制以及对精子利用和贮藏的影响。在附属腺蛋白中观察到的极端进化速率可能是由精子竞争和性冲动所驱动，这些过程最终可能驱动雌性和雄性分子与精子之间复杂的相互作用。但是，关于雌性生殖组织中的基因表达如何响应雄性分子和精子的变化而知之甚少。为了表征这种反应，我们对3天大未交配和交配的雌性果蝇黑腹果蝇的生殖道中的基因表达进行了平行的基因组和蛋白质组学分析。使用DNA芯片，我们鉴定了539个在未交配和交配的雌性中差异表达的转录本，并在交配后6 h的差异表达中出现了惊人的峰值，并且在交配后3 h内从最初的下调到主要上调的转录物有明显的转变。 。结合二维凝胶电泳和液相色谱质谱分析，我们在交配后3 h鉴定了84种差异表达的蛋白质，包括似乎经历了翻译后修饰的蛋白质。我们的观察共同定义了雌性生殖道中交配的转录和翻译反应，并提出了一种与雌性生殖道成熟相关的交配和最终阶段，随后与雄性生殖分子的衰落以及与之相关的双峰模型。精子的维护与利用。

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