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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Regulatory protein that inhibits both synthesis and use of the target protein controls flagellar phase variation in Salmonella enterica
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Regulatory protein that inhibits both synthesis and use of the target protein controls flagellar phase variation in Salmonella enterica

机译：抑制靶蛋白合成和使用的调节蛋白可控制肠沙门氏菌的鞭毛相变

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摘要

Flagellin is a major surface antigen for many bacterial species. The pathogen Salmonella enterica switches between two alternative, antigenic forms of its flagellin filament protein, either type B or C. This switching (flagellar phase variation) is achieved by stochastic inversion of a promoter that produces both type B flagellin (FljB) and an inhibitor (FljA) of type C flagellin formation. When the fljB-fljA operon is expressed, only type B flagella are produced; when the operon is not transcribed, the gene for type C flagellin (MC) is released from inhibition and forms type C flagella. Long thought to be a transcription repressor, the FljA inhibitor is shown here to block both translation and use of the FliC protein by binding to an mRNA region upstream from the translation start codon. Bypass mutants resistant to this inhibition alter this mRNA region, and some prevent FljA-RNA binding. Other bypass mutations are duplications within the leader mRNA that make FljA essential for FliC assembly. Certain bypass mutations allow FljA to block FliC-dependent motility without blocking production of the FliC protein, per se. Other mutations in the FliC mRNA leader block expression of the unlinked fljB gene. Results suggest that mRNAs for types B and C flagellin compete for occupancy of a site that directs the product toward assembly and that FljA influences this competition. This mechanism may serve to prevent assembly of flagella with a mixture of subunit types, especially during periods of switching from one type to the other.
机译：鞭毛蛋白是许多细菌物种的主要表面抗原。病原体小肠沙门氏菌在其鞭毛蛋白丝蛋白B或C的两种抗原性形式之间切换。这种切换（鞭毛相变）是通过随机产生产生B型鞭毛蛋白（FljB）和抑制剂的启动子来实现的（F1A）C型鞭毛蛋白形成。表达fljB-fljA操纵子时，仅产生B型鞭毛。当操纵子不被转录时，C型鞭毛蛋白（MC）的基因从抑制中释放出来并形成C型鞭毛。长期以来被认为是转录阻遏物的FljA抑制剂通过结合到翻译起始密码子上游的mRNA区域来阻止FliC蛋白的翻译和使用。对这种抑制具有抗性的旁路突变体会改变该mRNA区域，有些会阻止FljA-RNA结合。其他旁路突变是前导mRNA内的重复，使得FljA对于FliC组装至关重要。某些旁路突变本身允许FljA阻断FliC依赖的运动，而不会阻断FliC蛋白的产生。 FliC mRNA前导物中的其他突变会阻止未连接的fljB基因的表达。结果表明，B型和C型鞭毛蛋白的mRNA竞争着一个位点的占据，该位点将产物导向组装，而FljA影响了这种竞争。该机制可以用于防止鞭毛与亚基类型的混合物组装，尤其是在从一种类型转换为另一种类型的时期。

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