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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility
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Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility

机译：伯克霍尔德菌xenovorans LB400带有一个多复制子，9.73 Mbp基因组，具有多功能性

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摘要

Burkholderia xenovorans LB400 (LB400), a well studied, effective polychlorinated biphenyl-degrader, has one of the two largest known bacterial genomes and is the first nonpathogenic Burkholderia isolate sequenced. From an evolutionary perspective, we find significant differences in functional specialization between the three replicons of LB400, as well as a more relaxed selective pressure for genes located on the two smaller vs. the largest replicon. High genomic plasticity, diversity, and specialization within the Burkholderia genus are exemplified by the conservation of only 44% of the genes between LB400 and Burkholderia cepacia complex strain 383. Even among four B. xenovorans strains, genome size varies from 7.4 to 9.73 Mbp. The latter is largely explained by our findings that > 20% of the LB400 sequence was recently acquired by means of lateral gene transfer. Although a range of genetic factors associated with in vivo survival and intercellular interactions are present, these genetic factors are likely related to niche breadth rather than determinants of pathogenicity. The presence of at least eleven "central aromatic" and twenty "peripheral aromatic" pathways in LB400, among the highest in any sequenced bacterial genome, supports this hypothesis. Finally, in addition to the experimentally observed redundancy in benzoate degradation and formaldehyde oxidation pathways, the fact that 17.6% of proteins have a better LB400 paralog than an ortholog in a different genome highlights the importance of gene duplication and repeated acquirement, which, coupled with their divergence, raises questions regarding the role of paralogs and potential functional redundancies in large-genome microbes.
机译：伯克霍尔德菌xenovorans LB400（LB400）是一种经过充分研究的有效多氯联苯降解剂，具有两个最大的已知细菌基因组之一，并且是第一个测序的非致病性伯克霍尔德菌分离株。从进化的角度来看，我们发现LB400的三个复制子之间在功能专业化方面存在显着差异，并且对位于两个较小复制子与最大复制子上的基因的选择压力更加宽松。 LB400和洋葱伯克霍尔德菌复合体菌株383之间仅44％的基因保守性体现了伯克霍尔德菌属内的高基因组可塑性，多样性和专一性。即使在四个异食双歧杆菌中，基因组大小在7.4至9.73 Mbp之间变化。后者很大程度上由我们的发现所解释，即最近有超过20％的LB400序列是通过横向基因转移获得的。尽管存在与体内存活和细胞间相互作用相关的一系列遗传因素，但这些遗传因素可能与利基宽度有关，而不是致病性的决定因素。 LB400中至少11个“中央芳香族”和20个“周边芳香族”途径的存在，在任何测序的细菌基因组中最高，支持了这一假设。最后，除了实验观察到的苯甲酸酯降解和甲醛氧化途径的冗余外，在不同基因组中17.6％的蛋白质具有比直系同源物更好的LB400旁系同源性这一事实突出了基因复制和重复获取的重要性，再加上它们的差异，引发了关于旁系同源物的作用以及大基因组微生物中潜在功能冗余的问题。

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