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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Development of a specific system for targeting protein to metallophilic macrophages
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Development of a specific system for targeting protein to metallophilic macrophages

机译：开发将蛋白质靶向嗜金属巨噬细胞的特定系统

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摘要

The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL~+ cells). In naieve mice, these CRL~+ cells, metallophilic macrophages (Mφ) in spleen and subcapsular sinus Mφ in lymph nodes, are located strategically for antigen capture and are adjacent to B cell follicles, but their role in the immune response is unknown. We have exploited the lectin activity of CR to develop a highly specific system for targeting protein to CRL~+ Mφ. We demonstrate that the sulfated carbohydrates recognized by CR are exposed to the extracellular milieu and mediate highly specific targeting of CR-containing proteins. This model will allow the dissection of the role of metallophilic Mφ in an immune response in vivo.
机译：甘露糖受体的富含半胱氨酸的结构域（CR）结合由次级淋巴器官（CRL〜+细胞）中的髓样细胞亚群表达的硫酸化糖蛋白CR配体（CRL）。在幼稚的小鼠中，这些CRL〜+细胞，脾中的嗜金属巨噬细胞（Mφ）和淋巴结中的荚膜下窦Mφ在战略上位于抗原捕获位置，并且与B细胞滤泡相邻，但它们在免疫应答中的作用尚不清楚。我们已经利用CR的凝集素活性来开发一种高度特异性的系统，将蛋白质靶向CRL〜+Mφ。我们证明了被CR识别的硫酸化碳水化合物暴露于细胞外环境，并介导了含CR蛋白质的高度特异性靶向。该模型将剖析嗜金属Mφ在体内免疫反应中的作用。

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