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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling
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Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling

机译：串联网格对脑网格蛋白包被囊泡的MS分析显示它们在突触囊泡回收中的关键作用

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摘要

Tandem MS has identified 209 proteins of clathrin-coated vesicles (CCVs) isolated from rat brain. An overwhelming abundance of peptides were assigned to the clathrin coat with a 1:1 stoichiometry observed for clathrin heavy and light chains and a 2:1 stoichiometry of clathrin heavy chain with clathrin adaptor protein heterotetramers. Thirty-two proteins representing many of the known components of synaptic vesicles (SVs) were identified, supporting that a main function for brain CCVs is to recapture SVs after exocytosis. A ratio of vesicle-N-ethylmaleimide-sensitive factor attachment protein receptors to target-N-ethylmaleimide-sensitive factor attachment protein receptors, similar to that previously detected on SVs, supports a single-step model for SV sorting during CCV-mediated recycling of SVs. The uncovering of eight previously undescribed proteins, four of which have to date been linked to clathrin-mediated trafficking, further attests to the value of the current organelle-based proteomics strategy. [References: 50]
机译：串联质谱已鉴定出从大鼠脑中分离出的209种网格蛋白包被囊泡（CCV）蛋白。网格蛋白外壳分配了大量肽段，其中网格蛋白重链和轻链的化学计量比为1：1，网格蛋白衔接蛋白异四聚体的网格蛋白重链化学计量比为2：1。已鉴定出代表突触小泡（SVs）许多已知成分的32种蛋白质，支持脑CCV的主要功能是在胞吐作用后重新捕获SV。与先前在SV上检测到的相似，囊泡N-乙基马来酰亚胺敏感因子附着蛋白受体与靶N-乙基马来酰亚胺敏感因子附着蛋白受体的比率支持在CCV介导的CCV回收过程中SV分选的单步模型。 SV。揭示了八个先前未描述的蛋白质，迄今为止，其中四个已经与网格蛋白介导的运输有关，这进一步证明了当前基于细胞器的蛋白质组学策略的价值。 [参考：50]

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