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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Scanning electrochemical microscopy of menadione-glutathione conjugate export from yeast cells.
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Scanning electrochemical microscopy of menadione-glutathione conjugate export from yeast cells.

机译：甲萘醌-谷胱甘肽共轭物从酵母细胞中输出的扫描电化学显微镜。

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摘要

The uptake of menadione (2-methyl-1,4-naphthoquinone), which is toxic to yeast cells, and its expulsion as a glutathione complex were studied by scanning electrochemical microscopy. The progression of the in vitro reaction between menadione and glutathione was monitored electrochemically by cyclic voltammetry and correlated with the spectroscopic (UV-visible) behavior. By observing the scanning electrochemical microscope tip current of yeast cells suspended in a menadione-containing solution, the export of the conjugate from the cells with time could be measured. Similar experiments were performed on immobilized yeast cell aggregates stressed by a menadione solution. From the export of the menadione-glutathione conjugate detected at a 1-microm-diameter electrode situated 10 microm from the cells, a flux of about 30,000 thiodione molecules per second per cell was extracted. Numerical simulations based on an explicit finite difference method further revealed that the observation of a constant efflux of thiodione from the cells suggested the rate was limited by the uptake of menadione and that the efflux through the glutathione-conjugate pump was at least an order of magnitude faster.
机译：通过扫描电化学显微镜研究了对酵母细胞有毒的甲萘醌（2-甲基-1,4-萘醌）的摄取及其作为谷胱甘肽复合物的排出。通过循环伏安法电化学监测了甲萘醌和谷胱甘肽之间的体外反应进程，并与光谱（紫外可见）行为相关。通过观察悬浮在含有甲萘醌的溶液中的酵母细胞的扫描电化学显微镜尖端电流，可以测量缀合物随时间从细胞中的输出。对甲萘醌溶液胁迫的固定化酵母细胞聚集体进行了类似的实验。从在距细胞10微米处的直径为1微米的电极上检测到的甲萘醌-谷胱甘肽共轭物的输出中，每细胞每秒提取约30,000硫二酮分子的流量。基于显式有限差分法的数值模拟进一步表明，从细胞中不断观察到硫二酮的流出表明该速率受甲萘醌的摄取限制，并且通过谷胱甘肽-共轭泵的流出至少一个数量级。快点。

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