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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis.
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Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis.

机译：Camalexin由吲哚3-乙醛肟合成，这是拟南芥主要代谢和次要代谢之间的关键分支。

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摘要

Characteristic for cruciferous plants is their production of N- and S-containing indole phytoalexins with disease resistance and cancer-preventive properties, previously proposed to be synthesized from indole independently of tryptophan. We show that camalexin, the indole phytoalexin of Arabidopsis thaliana, is synthesized from tryptophan via indole-3-acetaldoxime (IAOx) in a reaction catalyzed by CYP79B2 and CYP79B3. Cyp79B2/cyp79B3 double knockout mutant is devoid of camalexin, as it is also devoid of indole glucosinolates [Zhao, Y., Hull, A. K., Gupta, N. R., Goss, K. A., Alonso, J., Ecker, J. R., Normanly, J., Chory, J. & Celenza, J. L. (2002) Genes Dev. 16, 3100-3112], and isotope-labeled IAOx is incorporated into camalexin. These results demonstrate that only CYP79B2 and CYP79B3 contribute significantly to the IAOx pool from which camalexin and indole glucosinolates are synthesized. Furthermore, production of camalexin in the sur1 mutant devoid of glucosinolates excludes the possibility that camalexin is derived from indole glucosinolates. CYP79B2 plays an important role in camalexin biosynthesis in that the transcript level of CYP79B2, but not CYP79B3, is increased upon induction of camalexin by silver nitrate as evidenced by microarray analysis and promoter-beta-glucuronidase data. The structural similarity between cruciferous indole phytoalexins suggests that these compounds are biogenetically related and synthesized from tryptophan via IAOx by CYP79B homologues. The data show that IAOx is a key branching point between several secondary metabolic pathways as well as primary metabolism, where IAOx has been shown to play a critical role in IAA homeostasis.
机译：十字花科植物的特征是它们产生具有抗病性和癌症预防特性的含N和S的吲哚植物抗毒素，以前建议由吲哚独立于色氨酸合成。我们显示camalexin，拟南芥的吲哚植物抗毒素，是通过CYP79B2和CYP79B3催化的反应通过色氨酸通过吲哚-3-乙醛肟（IAOx）合成的。 Cyp79B2 / cyp79B3双敲除突变体不含camalexin，因为它也不含吲哚芥子油苷[Zhao，Y.，Hull，AK，Gupta，NR，Goss，KA，Alonso，J.，Ecker，JR，Normanly，J.。 ，Chory，J。＆Celenza，JL（2002）Genes Dev。 16，16，3100-3112]，并将同位素标记的IAOx并入camalexin。这些结果表明，只有CYP79B2和CYP79B3对IAOx库有显着贡献，从中合成了卡莱姆辛和吲哚芥子油苷。此外，在没有硫代芥子油苷的sur1突变体中产生camalexin排除了camalexin衍生自吲哚芥子油苷的可能性。 CYP79B2在camalexin生物合成中起着重要作用，如微阵列分析和启动子-β-葡糖醛酸苷酸酶数据所证明，CYP79B2而不是CYP79B3的转录水平在硝酸银诱导camalexin时增加。十字花科吲哚植物抗毒素之间的结构相似性表明，这些化合物是生物遗传相关的，并且是通过色氨酸经由CYP79B同源物通过IAOx从色氨酸合成的。数据表明，IAOx是几个次级代谢途径和初级代谢之间的关键分支点，其中IAOx已显示在IAA稳态中起关键作用。

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