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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Transcriptional repression of target genes by LEUNIG and SEUSS, two interacting regulatory proteins for Arabidopsis flower development.
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Transcriptional repression of target genes by LEUNIG and SEUSS, two interacting regulatory proteins for Arabidopsis flower development.

机译：LEUNIG和SEUSS是拟南芥花发育的两种相互作用的调节蛋白，可抑制目标基因的转录。

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摘要

Transcription repression plays important roles in preventing crucial regulatory proteins from being expressed in inappropriate temporal or spatial domains. LEUNIG (LUG) and SEUSS (SEU) normally act to prevent ectopic expression of the floral homeotic gene AGAMOUS in flowers. LUG encodes a protein with sequence similarities to the yeast Tup1 corepressor. SEU encodes a plant-specific regulatory protein with sequence similarity in a conserved dimerization domain to the LIM-domain binding 1/Chip proteins in mouse and Drosophila. Despite the molecular isolation of LUG and SEU, the biochemical function of these two proteins remains uncharacterized, and the mechanism of AGAMOUS repression remains unknown. Here, we report that LUG and SEU interact directly in vitro and in vivo. Furthermore, LUG exhibits a strong repressor activity on several heterologous promoters in yeast and plant cells. SEU, in contrast, does not exhibit any direct repressor activity, but can repress reporter gene expression only in the presence of LUG, indicating a possible role of SEU as an adaptor protein for LUG. Our results demonstrate that LUG encodes a functional homologue of Tup1 and that SEU may function similarly to Ssn6, an adaptor protein of Tup1. We have defined the LUG/LUH, Flo8, single-strand DNA-binding protein domain of LUG as both necessary and sufficient for the interaction with SEU and two domains of LUG as important for its repressor function. Our work provides functional insights into plant transcriptional corepressors and reveals both conservation and distinctions between plant corepressors and those of yeast and animals.
机译：转录抑制在防止关键调控蛋白在不适当的时域或空间域中表达中起着重要作用。 LEUNIG（LUG）和SEUSS（SEU）通常用来防止花朵上的花卉同源基因AGAMOUS异位表达。 LUG编码一种与酵母Tup1 corepressor具有序列相似性的蛋白质。 SEU编码一种植物特异性调节蛋白，在保守的二聚化域中与小鼠和果蝇中的LIM域结合1 / Chip蛋白具有序列相似性。尽管将LUG和SEU进行了分子分离，但这两种蛋白的生化功能仍未表征，并且对AGAMOUS的抑制作用机制仍然未知。在这里，我们报道LUG和SEU在体外和体内直接相互作用。此外，LUG对酵母和植物细胞中的几种异源启动子表现出强大的阻遏活性。相反，SEU没有任何直接的阻遏物活性，但是只有在LUG存在的情况下，SEU才能抑制报告基因的表达，这表明SEU可能是LUG的衔接蛋白。我们的结果表明，LUG编码Tup1的功能同源物，并且SEU的功能可能类似于Tup1的衔接蛋白Ssn6。我们已经定义了LUG的LUG / LUH，Flo8，单链DNA结合蛋白结构域与SEU的相互作用既必要且足够，而LUG的两个结构域对其阻遏物功能也很重要。我们的工作提供了对植物转录共表达的功能性见解，并揭示了植物共表达和酵母和动物的保守性和区别。

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