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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Quantitative proteomic analysis indicates increased synthesis of a quinolone by Pseudomonas aeruginosa isolates from cystic fibrosis airways.
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Quantitative proteomic analysis indicates increased synthesis of a quinolone by Pseudomonas aeruginosa isolates from cystic fibrosis airways.

机译：定量蛋白质组学分析表明，来自囊性纤维化气道的铜绿假单胞菌分离物对喹诺酮的合成增加。

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摘要

The opportunistic bacterial pathogen Pseudomonas aeruginosa colonizes airways of individuals with cystic fibrosis (CF) with resultant chronic destructive lung disease. P. aeruginosa adaptation to the CF airway includes biofilm formation and antibiotic resistance. Isolates from asymptomatic individuals in the first 3 years of life have unique characteristics, suggesting that adaptation occurs before clinical symptoms. One defined early adaptation is expression of a specific proinflammatory lipopolysaccharide (LPS) that is associated with antimicrobial peptide resistance. This CF-specific LPS is induced when P. aeruginosa is grown in medium that is limited for magnesium. Therefore, qualitative and quantitative proteomic approaches were used to define 1,331 P. aeruginosa proteins, of which 145 were differentially expressed on limitation of magnesium. Among proteins induced by low magnesium were enzymes essential for production of 2-heptyl 3-hydroxy 4-quinolone, the Pseudomonas quinolone signal (PQS), which interacts with the homoserine lactone signaling pathway. Measurement of PQS in P. aeruginosa isolates from asymptomatic children with CF indicated that strains with increased synthesis of PQS are present during early colonization of CF patient airways.
机译：机会性细菌病原菌铜绿假单胞菌定植于患有囊性纤维化（CF）并导致慢性破坏性肺病的个体的气道中。铜绿假单胞菌对CF气道的适应性包括生物膜形成和抗生素抗性。在生命的最初3年中，无症状个体的分离株具有独特的特征，这表明适应发生在临床症状发生之前。一种定义的早期适应是与抗菌肽耐药性相关的特定促炎性脂多糖（LPS）的表达。当铜绿假单胞菌生长在镁受限的培养基中时，会诱导CF特异性LPS。因此，使用定性和定量蛋白质组学方法定义了1,331个铜绿假单胞菌蛋白质，其中145个由于镁的限制而差异表达。在低镁诱导的蛋白质中，对于产生2-庚基3-羟基4-喹诺酮（假单胞菌喹诺酮信号（PQS），与高丝氨酸内酯信号传导途径相互作用）必需的酶。对无症状CF儿童的铜绿假单胞菌分离物中PQS的测量表明，在CF患者气道的早期定殖过程中，存在PQS合成增加的菌株。

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