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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.
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The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.

机译：中国仓鼠二氢叶酸还原酶复制起点中的基质附着区域可能是局部染色单体分离所必需的。

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摘要

Centered in the Chinese hamster dihydrofolate reductase origin of replication is a prominent nuclear matrix attachment region (MAR). Indirect lines of evidence suggested that this MAR might be required for origin activation in early S phase. To test this possibility, we have deleted the MAR from a Chinese hamster ovary variant harboring a single copy of the dihydrofolate reductase locus. However, 2D gel replicon mapping shows that removal of the MAR has no significant effect either on the frequency or timing of initiation in this locus. Rather, fluorescence in situ hybridization studies on cells swollen under either neutral or alkaline conditions show that deletion of the MAR interferes with local separation of daughter chromatids. This surprising result provides direct genetic evidence that at least a subset of MARs performs an important biological function, possibly related to chromatid cohesion and separation.
机译：以中国仓鼠二氢叶酸还原酶为复制中心的是一个突出的核基质附着区（MAR）。间接证据表明，此MAR可能是S期早期激活起源所必需的。为了测试这种可能性，我们从具有单个拷贝的二氢叶酸还原酶基因座的中国仓鼠卵巢变体中删除了MAR。但是，二维凝胶复制子作图显示，去除MAR对该位点的启动频率或启动时间均无明显影响。相反，对在中性或碱性条件下肿胀的细胞进行的荧光原位杂交研究表明，MAR的缺失会干扰子代染色单体的局部分离。这一令人惊讶的结果提供了直接的遗传学证据，表明至少一个MAR子集具有重要的生物学功能，可能与染色单体的内聚和分离有关。

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