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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Comprehensive sampling of gene expression in human cell lines with massively parallel signature sequencing.
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Comprehensive sampling of gene expression in human cell lines with massively parallel signature sequencing.

机译：使用大规模平行签名测序对人类细胞系中的基因表达进行全面采样。

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摘要

Whereas information is rapidly accumulating about the structure and position of genes encoded in the human genome, less is known about the complexity and relative abundance of their expression in individual human cells and tissues. Here, we describe the characteristics of the transcriptomes of two cultured cell lines, HB4a (normal breast epithelium) and HCT-116 (colon adenocarcinoma), using massively parallel signature sequencing (MPSS). We generated in excess of 10(7) short signature sequences per cell line, thus providing a comprehensive snapshot of gene expression, within the technical limitations of the method. The number of genes expressed at one copy per cell or more in either of the lines was estimated to be between 10,000 and 15,000. The vast majority of the transcripts found in these cells can be mapped to known genes and their polyadenylation variants. Among the genes that could be identified from their signature sequences, approximately 8,500 were expressed by both cell lines, whereas 6,000 showed cellular specificity. Taking into account sequence tags that map uniquely to the genome but not to known transcripts, overall the data are consistent with an upper limit of 17,000 for the total number of genes expressed at more than one copy per cell in one or both of the two cell lines examined.
机译：尽管有关人类基因组中编码的基因的结构和位置的信息正在迅速积累，但人们对其在单个人类细胞和组织中表达的复杂性和相对丰度的了解却很少。在这里，我们使用大规模并行签名测序（MPSS）描述了两种培养的细胞系HB4a（正常乳腺上皮细胞）和HCT-116（结肠腺癌）的转录组特征。我们在每个细胞系中生成了10（7）个以上的短签名序列，从而在该方法的技术限制内提供了基因表达的全面快照。在任一细胞系中每个细胞以一个或多个拷贝表达的基因数量估计在10,000和15,000之间。在这些细胞中发现的绝大多数转录物都可以定位到已知的基因及其聚腺苷酸化变体。在可以从其签名序列中鉴定出的基因中，两种细胞系均表达了约8,500个基因，而6,000个则显示出细胞特异性。考虑到唯一映射到基因组但不映射到已知转录本的序列标签，总体而言，数据与两个细胞中一个或两个中每个细胞以一个以上拷贝表达的基因总数的上限17,000一致线检查。

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