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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution.
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TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution.

机译：通过进化，TRAP230 / ARC240和TRAP240 / ARC250介体亚基在功能上是保守的。

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摘要

In Saccharomyces cerevisiae Mediator, a subgroup of proteins (Srb8, Srb9, Srb10, and Srb11) form a module, which is involved in negative regulation of transcription. Homologues of Srb10 and Srb11 are found in some mammalian Mediator preparations, whereas no clear homologues have been reported for Srb8 and Srb9. Here, we identify a TRAP240/ARC250 homologue in Schizosaccharomyces pombe and demonstrate that this protein, spTrap240, is stably associated with a larger form of Mediator, which also contains conserved homologues of Srb8, Srb10, and Srb11. We find that spTrap240 and Sch. pombe Srb8 (spSrb8) regulate the same distinct subset of genes and have indistinguishable phenotypic characteristics. Importantly, Mediator containing the spSrb8/spTrap240/spSrb10/spSrb11 subunits is isolated only in free form, devoid of RNA polymerase II. In contrast, Mediator lacking this module associates with the polymerase. Our findings provide experimental evidence for recent suggestions that TRAP230/ARC240 and TRAP240/ARC250 may indeed be the Srb8 and Srb9 homologues of mammalian Mediator. Apparently Srb8/TRAP230/ARC240, Srb9/TRAP240/ARC250, Srb10, and Srb11 constitute a conserved Mediator submodule, which is involved in negative regulation of transcription in all eukaryotes.
机译：在酿酒酵母介体中，蛋白质的一个亚组（Srb8，Srb9，Srb10和Srb11）形成一个模块，该模块参与转录的负调控。在某些哺乳动物介体制剂中发现了Srb10和Srb11的同系物，而Srb8和Srb9的同系物尚未见报道。在这里，我们鉴定了粟酒裂殖酵母中的TRAP240 / ARC250同源物，并证明了该蛋白spTrap240与较大形式的介体稳定相关，该介体也包含Srb8，Srb10和Srb11的保守同源物。我们发现spTrap240和Sch。 pombe Srb8（spSrb8）调节相同的不同基因子集，并且具有难以区分的表型特征。重要的是，仅以游离形式分离出包含spSrb8 / spTrap240 / spSrb10 / spSrb11亚基的介体，没有RNA聚合酶II。相反，缺乏该模块的介体与聚合酶缔合。我们的发现为最近提出的TRAP230 / ARC240和TRAP240 / ARC250确实是哺乳动物介体的Srb8和Srb9同源物提供了实验证据。显然，Srb8 / TRAP230 / ARC240，Srb9 / TRAP240 / ARC250，Srb10和Srb11构成保守的介体子模块，该模块参与所有真核生物的转录负调控。

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